The ERM-1 membrane-binding domain directs erm-1 mRNA localization to the plasma membrane in the C. elegans embryo.
The ERM-1 membrane-binding domain directs erm-1 mRNA localization to the plasma membrane in the C. elegans embryo.
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ERM-1膜结合结构域将ERM-1 mRNA定位定位到秀丽隐杆线虫胚胎中的质膜。
DOI：
10.1242/dev.200930
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发表时间：
2022-11-15
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mRNA的定位和转运是调控基因表达不可或缺的环节。在秀丽隐杆线虫胚胎中，母系遗传的mRNA erm-1 （Ezrin/Radixin/Moesin）集中在前卵裂球中。erm-1 mRNA在这些卵裂球内定位到质膜，在质膜中也发现了必需的erm-1蛋白，一种膜-肌动蛋白连接物。我们证明了erm-1 mRNA在质膜上的定位依赖于翻译，并且需要其编码的n端膜结合（FERM）结构域。通过多种方法干扰翻译，我们发现只有当新生肽与翻译mRNA保持复合物时，erm-1 mRNA在质膜上的定位才会持续。事实上，在保留编码氨基酸序列的同时，重新编码erm-1 mRNA编码序列并没有破坏erm-1 mRNA的定位，这证实了指导mRNA定位的信息存在于其膜结合蛋白结构域内。对编码相似膜结合结构域的17个基因进行单分子廉价荧光原位杂交筛选，在早期胚胎中鉴定出3个质膜定位mrna。另外10个转录本在发育后期显示出潜在的膜定位。这些发现指出了编码膜相关蛋白的mrna定位的翻译依赖途径。摘要：在秀丽隐杆线虫中，erm-1 mRNA在质膜上的定位需要erm-1膜结合域。
mRNA localization and transport are integral in regulating gene expression. In Caenorhabditis elegans embryos, the maternally inherited mRNA erm-1 (Ezrin/Radixin/Moesin) becomes concentrated in anterior blastomeres. erm-1 mRNA localizes within those blastomeres to the plasma membrane where the essential ERM-1 protein, a membrane-actin linker, is also found. We demonstrate that the localization of erm-1 mRNA to the plasma membrane is translation dependent and requires its encoded N-terminal, membrane-binding (FERM) domain. By perturbing translation through multiple methods, we found that erm-1 mRNA localization at the plasma membrane persisted only if the nascent peptide remained in complex with the translating mRNA. Indeed, re-coding the erm-1 mRNA coding sequence while preserving the encoded amino acid sequence did not disrupt erm-1 mRNA localization, corroborating that the information directing mRNA localization resides within its membrane-binding protein domain. A single-molecule inexpensive fluorescence in situ hybridization screen of 17 genes encoding similar membrane-binding domains identified three plasma membrane-localized mRNAs in the early embryo. Ten additional transcripts showed potential membrane localization later in development. These findings point to a translation-dependent pathway for localization of mRNAs encoding membrane-associated proteins. Summary: In C. elegans, erm-1 mRNA localization to plasma membranes requires the ERM-1 membrane-binding domain.