Sensitive detection of a plant virus by electrochemical enzyme-linked immunoassay
Sensitive detection of a plant virus by electrochemical enzyme-linked immunoassay
复制标题
DOI：
10.1007/s002160000423
复制
发表时间：
2000-07
期刊：
影响因子：
--
通讯作者：
K. Jiao;Wei Sun;Shu-Sheng Zhang
中科院分区：
文献类型：
--
作者：
K. Jiao;Wei Sun;Shu-Sheng Zhang
电化学酶联免疫分析法检测黄瓜花叶病毒（CMV）的灵敏度比分光光度法（OPD）酶联免疫吸附试验（ELISA）提高了5倍。CMV的检测限为1.0 ng/mL，感染叶片汁液的最高稀释比为1：5.0 × 10 ~ 4。 该方法是基于偶联HRP-IgG偶联物催化邻氨基苯酚（OAP）-H2 O2氧化反应和酶产物的电还原反应。在pH8.0的Britton-Robinson（B-R）缓冲溶液中，酶解产物2-氨基吩恶嗪-3-酮在-0.65V（vs. Ag/AgCl）处有灵敏的二阶导数线性扫描伏安响应。因此，该方法可用于植物病毒的检测，并可大大提高检测的灵敏度。
The electrochemical enzyme-linked immunoassay increases the sensitivity of the detection of cucumber mosaic virus (CMV) by 5-fold compared with the spectrophotometric o-phenylenediamine (OPD) enzyme-linked immunosorbent assay (ELISA). The detection limit for the purified CMV is 1.0 ng/mL and the highest dilution ratio of the infected leaf sap is 1 : 5.0 × 104. The method is based on coupling the oxidation reaction of o-aminophenol (OAP)-H2O2catalyzed by HRP-IgG conjugate with the electro-reduction of the enzymatic product. The enzymatic product 2-aminophenoxazine-3-one exhibits a sensitive second order derivative linear-sweep voltammetric response at the potential of –0.65 V (vs. Ag/AgCl) in pH 8.0 Britton-Robinson (B-R) buffer solution. So it can be applied to the detection of the plant virus with highly improved sensitivity.