Development of a quantitative, portable, and automated fluorescent blue-ray device-based malaria diagnostic equipment with an on-disc SiO2 nanofiber filter
Development of a quantitative, portable, and automated fluorescent blue-ray device-based malaria diagnostic equipment with an on-disc SiO2 nanofiber filter
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DOI：
10.1038/s41598-020-63615-2
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发表时间：
2020-04-20
影响因子：
4.6
通讯作者：
Mita, Toshihiro
中科院分区：
文献类型：
--
作者：
Yamamoto, Takeki;Hashimoto, Muneaki;Mita, Toshihiro
迫切需要开发一种疟疾自动诊断系统，该系统可以容易且快速地检测疟疾寄生虫并确定临床血液样品中疟疾感染的红细胞的比例。在这项研究中，我们开发了一个定量的，移动的，全自动疟疾诊断系统，配备了一个光盘上的二氧化硅滤光片和蓝光设备。过滤器从血液样本中去除白细胞和血小板，这会干扰蓝光设备对疟疾的准确检测。我们证实，该过滤器可以通过圆盘旋转产生的离心力自动操作，在短短30秒内实现了白细胞（99.7%）和血小板（90.2%）的高去除率。自动化系统表现出更高的灵敏度（100%）和特异性（92.8%）检测恶性疟原虫从血液中的274个无症状的人在肯尼亚相比，常见的快速诊断测试（灵敏度=98.1%，特异性= 54.8%）。这表明，该系统可以替代缺乏基本基础设施的地方卫生设施使用的传统方法。
There is an urgent need to develop an automated malaria diagnostic system that can easily and rapidly detect malaria parasites and determine the proportion of malaria-infected erythrocytes in the clinical blood samples. In this study, we developed a quantitative, mobile, and fully automated malaria diagnostic system equipped with an on-disc SiO2 nanofiber filter and blue-ray devices. The filter removes the leukocytes and platelets from the blood samples, which interfere with the accurate detection of malaria by the blue-ray devices. We confirmed that the filter, which can be operated automatically by centrifugal force due to the rotation of the disc, achieved a high removal rate of leukocytes (99.7%) and platelets (90.2%) in just 30s. The automated system exhibited a higher sensitivity (100%) and specificity (92.8%) for detecting Plasmodium falciparum from the blood of 274 asymptomatic individuals in Kenya when compared to the common rapid diagnosis test (sensitivity=98.1% and specificity = 54.8%). This indicated that this system can be a potential alternative to conventional methods used at local health facilities, which lack basic infrastructure.