Preferential replication of FIV in activated CD4+CD25+T cells independent of cellular proliferation
Preferential replication of FIV in activated CD4+CD25+T cells independent of cellular proliferation
复制标题
DOI：
10.1016/j.virol.2004.01.014
复制
发表时间：
2004-04-10
期刊：
影响因子：
3.7
通讯作者：
Tompkins, MB
中科院分区：
文献类型：
--
作者：
Joshi, A;Vahlenkamp, TW;Tompkins, MB
试图确定HIV-1潜伏期的研究已经证明，该病毒在CD 4+细胞亚群中作为潜伏性和生产性感染持续存在。然而，关于在体外静止T细胞中建立稳定的HIV-1感染的报道是有争议的。在本研究中，我们研究了初始和活化的CD 4（+）细胞亚群（以CD 25的差异表达区分）对猫免疫缺陷病毒（FIV）感染的易感性，它们复制病毒的能力，以及可能作为病毒在感染动物中持续存在的储存库。虽然CD 4（+）CD 25（+）和CD 4（+）CD 25（-）细胞在体外和体内都对FIV感染敏感，但当与白细胞介素-2（IL-2）一起培养时，只有CD 4（+）CD 25（+）细胞产生感染性病毒体。潜伏感染的CD 4（+）CD 25（-）细胞在伴刀豆素A（ConA）刺激后产生感染性病毒体，其与上调的CD 25表面表达相关。与CD 4（+）CD 25（-）细胞相反，CD 4（+）CD 25（+）细胞对有丝分裂原刺激无反应，并且无论是否感染FIV，对凋亡都具有相对抗性。CD 4（+）CD 25（+）细胞在IL-2存在下有效复制FIV的能力，但仍然对凋亡信号无反应性和无反应性，这表明这些细胞可能提供了生产性FIV感染的储存库。相反，CD 4（+）CD 25（-）细胞似乎建立了潜伏的病毒库，能够在刺激后重新激活。(C)2004年爱思唯尔公司All rights reserved.
Studies attempting to identify reservoirs of HIV-1 latency have documented that the virus persists as both a latent and productive infection in subsets of CD4+ cells. Reports regarding establishment of a stable HIV-1 infection in quiescent T cells in vitro, however, are controversial. In the present study, we investigated the susceptibility of naive and activated CD4(+) cell subsets (distinguished by differential expression of CD25) to feline immunodeficiency virus (FIV) infection, their ability to replicate the virus, and potentially act as a reservoir for virus persistence in infected animals. While both CD4(+)CD25(+) and CD4(+)CD25(-) cells are susceptible to FIV infection in vitro and in vivo, only CD4(+)CD25(+) cells produce infectious virions when cultured with interleukin-2 (IL-2). Latently infected CD4(+)CD25(-) cells produce infectious virions following ConcanvalinA (ConA) stimulation, which correlates with upregulated surface expression of CD25. In contrast to CD4(+)CD25(-) cells, CD4(+)CD25(+) cells remain unresponsive to mitogen stimulation and are relatively resistant to apoptosis whether or not infected with FIV. The ability of CD4(+)CD25(+) cells to replicate FIV efficiently in the presence of IL-2 but remain anergic and unresponsive to apoptotic signaling suggests that these cells may provide a reservoir of productive FIV infection. On the contrary, CD4(+)CD25(-) cells seem to establish as latent viral reservoirs capable of being reactivated after stimulation. (C) 2004 Elsevier Inc. All rights reserved.