Immunoassay with Single-Walled Carbon Nanotubes as Near-Infrared Fluorescent Labels
Immunoassay with Single-Walled Carbon Nanotubes as Near-Infrared Fluorescent Labels
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DOI：
10.1021/am401702q
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发表时间：
2013-08-28
影响因子：
9.5
通讯作者：
Yudasaka, Masako
中科院分区：
文献类型：
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作者：
Iizumi, Yoko;Okazaki, Toshiya;Yudasaka, Masako
单壁碳纳米管（CNTs）在1000 nm以上的近红外（NIR）区域内的固有光致发光特性使其具有低生物有机分子干扰和深组织穿透性，成为生物探针的理想候选材料。我们在这里展示了一种免疫测定法，通过使用近红外CNT标记缀合到免疫球蛋白G（IgG）抗体。大多数CNT缀合的IgG成功地与蛋白G连接的磁珠免疫沉淀并从其洗脱，这通过缀合的CNT在1000-1200 nm处的NIR发射证实。CNT标记物的光致发光强度足够强以通过我们的简单程序检测600 pM的抗原。
The intrinsic photoluminescence of single-walled carbon nanotubes (CNTs) in the near-infrared (NIR) above 1000 nm makes them promising candidates for biological probes owing to low interference by bioorganic molecules and deep tissue penetration. We here demonstrate an immunoassay by using a NIR CNT labels conjugated to immunoglobulin G (IgG) antibodies. Most of the CNT-conjugated IgG was successfully immunoprecipitated with protein G-attached magnetic beads and eluted from them, which was confirmed by the NIR emission of the conjugated CNTs at 1000-1200 nm. The photoluminescence intensity of the CNT labels was strong enough to detect antigens at 600 pM by our simple procedures.