Effects of anticoagulants on Plasmodium vivax oocyst development in Anopheles albimanus mosquitoes
Effects of anticoagulants on Plasmodium vivax oocyst development in Anopheles albimanus mosquitoes
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DOI：
10.4269/ajtmh.2007.77.242
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发表时间：
2007-08-01
影响因子：
3.3
通讯作者：
Arevalo-Herrera, Myriam
中科院分区：
文献类型：
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作者：
Solarte, Yezid;Manzano, Maria del Rosario;Arevalo-Herrera, Myriam
采用人工膜饲养法（AMF）测定按蚊阻断疟疾传播的活性.本研究的目的是确定最广泛使用的抗凝剂EDTA和肝素对间日疟原虫孢子生殖周期发育的影响。从60名携带间日疟原虫感染的患者收集的血液样品用于喂养An。利用AMF.喂食后7天，解剖蚊子以评估蚊子感染。用含EDTA的血液喂养的蚊子显示出较低的平均卵囊数，与用肝素喂养的血液相比。然而，当血清中EDTA浓度降低时，这种影响最小化。这一结果可能是由于小配子体需要Ca ~（2+）、Mn ~（2+）和Mg ~（2+）来激活对出丝过程和动合子运动性很重要的酶。因此，我们建议使用肝素作为AMF检测中血液的抗凝剂。
Artificial membrane feeding (AMF) assays are used to determine malaria transmission- blocking activity in Anopheles. The purpose of this study was to determine the effect of the most widely used anticoagulants, EDTA and heparin, on development of the Plasmodium vivax sporogonic cycle. Blood samples collected from 60 patients carrying P. vivax infections were used to feed An. albimanus usin g AMF. Seven days after feeding, mosquitoes were dissected to assess mosquito infection. Mosquitoes fed with blood containing EDTA showed a lower mean oocyst number as compared with those fed blood with heparin. However, this effect was minimized upon reduction of EDTA concentrations in the serum. This result may be explained by the fact that microgametocytes require Ca2+, Mn2+, and Mg+2 to activate enzymes important for exflagellation process and for motility of ookinetes. We therefore recommend that heparin be used as the anticoagulant of choice for blood used in AMF assays.