Self-associating IgG rheumatoid factors in MRL/l autoimmune mice.
Self-associating IgG rheumatoid factors in MRL/l autoimmune mice.
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MRL/l 自身免疫小鼠中的自关联 IgG 类风湿因子。
DOI：
10.1002/art.1780271013
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发表时间：
1984
影响因子：
--
通讯作者：
Mannik,M
中科院分区：
文献类型：
--
作者：
Nardella,FA;Teller,DC;Izui,S;Mannik,M
先前的工作表明，从类风湿关节炎患者血浆中分离的中间复合物由自缔合IgG类风湿因子组成。MRL/1品系的小鼠发展为具有关节炎的自发性自身免疫性疾病，其在病理学上类似于人类类风湿性关节炎。此外，MRL/1小鼠的血清含有针对核抗原的自身抗体以及IgM和IgG类风湿因子。本研究旨在确定从这些小鼠中分离的IgG类风湿因子是否发生自缔合。根据血清-血清沉淀素相互作用将MRL/1小鼠血清分为A组和B组。用沉降速率超离心法测定了13份MRL/1小鼠血清中13份含有正常血清6.6S和19 S组分之间的中间复合物。A组和B组之间的中间复合物水平无差异。从其他9只小鼠的血清中分离IgG类风湿因子。在沉降平衡超离心后，这些类风湿因子经历了与人自缔合IgG类风湿因子相似的浓度依赖性自缔合，尽管无法确定自缔合的精确化学计量。B组IgG类风湿因子的自相互作用能量高于A组。这些研究提供了额外的证据，MRL/1小鼠可能是研究人类类风湿性关节炎的最佳动物模型。
Previous work has shown that the intermediate complexes isolated from the plasma of patients with rheumatoid arthritis are composed of self‐associating IgG rheumatoid factors. Mice of the MRL/1 strain develop spontaneous autoimmune disease with arthritis that is pathologically similar to human rheumatoid arthritis. Also, the sera of MRL/1 mice contain autoantibodies to nuclear antigens as well as IgM and IgG rheumatoid factors. The present studies were done to determine if the IgG rheumatoid factors isolated from these mice undergo self‐association. MRL/1 mouse sera were categorized into groups A and B based on serumserum precipitin interactions. Thirteen of 13 MRL/1 mice sera examined contained intermediate complexes sedimenting between the 6.6S and 19S components of normal serum by sedimentation velocity ultracentrifugation. There were no differences in the level of intermediate complexes between groups A and B. IgG rheumatoid factors were isolated from the sera of 9 other mice. Upon sedimentation equilibrium ultracentrifugation, these rheumatoid factors underwent concentration‐dependent self‐association similar to that described for human self‐associating IgG rheumatoid factors, although the precise stoichiometry of self‐association could not be determined. The IgG rheumatoid factors from group B had higher energies of self‐interaction than those from group A. These studies provide additional evidence that MRL/1 mice may be the best available animal model for the study of human rheumatoid arthritis.