Electrophoretic and serological characterization of the lipopolysaccharide produced by Neisseria gonorrhoeae.
Electrophoretic and serological characterization of the lipopolysaccharide produced by Neisseria gonorrhoeae.
复制标题
淋病奈瑟菌产生的脂多糖的电泳和血清学表征。
DOI：
10.1093/infdis/149.4.544
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Morse,SA
中科院分区：
文献类型：
--
作者：
Mintz,CS;Apicella,MA;Morse,SA
用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对淋病奈瑟菌脂多糖（LPS）进行纯化，结果显示多条带。许多条带由LPS聚集体组成，用0.1MNaOH处理或加入4 Murea到分离胶中可将其解离。未聚集的LPS通常在朝向凝胶底部的一到三个条带中发现，结果表明淋球菌LPS上不存在长重复的0抗原。不同血清型淋病奈瑟菌LPS的SDS-PAGE分析表明，LPS存在结构异质性。用酶联免疫吸附试验抑制用苯酚-氯仿-石油醚（PCP）和苯酚-水提取的淋球菌LPS的抗原分析显示，PCP提取的LPS含有比苯酚-水提取的LPS少得多的淋球菌型特异性抗原。结果表明，PCP法和酚水法提取的淋病奈瑟菌LPS分子种类不同。
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of purified lipopolysaccharide (LPS) fromNeisseria gonorrhoeaeresulted in the formation of multiple bands. Many of the bands consisted of LPS aggregates, which could be dissociated by treatment with 0.1MNaOH or by addition of 4Murea to the separating gel. The unaggregated LPS was usually found in one to three bands toward the bottom of the gels, a result suggesting that a long repeating 0 antigen is not present on gonococcal LPS. SDS-PAGE of LPS from different LPS serotypes ofN gonorrhoeaeindicated that structural heterogeneity exists. Antigenic analysis by enzyme-linked immunosorbent assay inhibition of gonococcal LPS extracted with phenol-chloroform-petroleum ether (PCP) and phenol-water revealed that PCP-extracted LPS contained substantially less serotype-specific antigen than did phenol-water-extracted LPS. These results suggest that the PCP and phenol-water methods extract different molecular species of LPS fromN gonorrhoeae.