ORGANIZATION AND NUCLEOTIDE-SEQUENCE OF A NEW RIBOSOMAL OPERON IN ESCHERICHIA-COLI CONTAINING THE GENES FOR RIBOSOMAL-PROTEIN S2 AND ELONGATION-FACTOR TS
ORGANIZATION AND NUCLEOTIDE-SEQUENCE OF A NEW RIBOSOMAL OPERON IN ESCHERICHIA-COLI CONTAINING THE GENES FOR RIBOSOMAL-PROTEIN S2 AND ELONGATION-FACTOR TS
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DOI：
10.1093/nar/9.16.4163
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发表时间：
1981-01-01
影响因子：
14.9
通讯作者：
FRIESEN, JD
中科院分区：
文献类型：
--
作者：
AN, G;BENDIAK, DS;FRIESEN, JD
本文报道了包括核糖体蛋白S2（rpsB）和翻译延伸因子EF-Ts（tsf）基因在内的基因组遗传图谱的四个最小区域的核苷酸序列，以及这两个基因操纵子中调控位点的可能位置。结果表明，基因顺序为：rpsBp-rpsB-tsf-tsft。一个潜在的调节位点是在包括核糖体结合位点和翻译起始密码子的herpsB前导区中的16个核苷酸序列。这与16 S核糖体RNA 5′端的核苷酸8至23具有高度的同源性，可能意味着一个序列是转录后控制rpsB表达所必需的。这些数据允许人们推断S2和EF-Ts的氨基酸序列。
We report the nucleotide sequence of the four min region of theEscherichiacoligenetic map that includes the genes for ribosomal protein S2 (rpsB) and translation elongation factor EF-Ts (tsf), and the possible location of regulatory sites within this two gene operon. The data indicate that the gene order is:rpsBp-rpsB-tsf-tsft. One potential regulatory site is a 16 nucleotide sequence in therpsBleader region encompassing the ribosome binding site and the translation initiation codon. This has a high degree of homology with nucleotides 8 through 23 on the 5′ end of 16S ribosomal RNA, and might signify a sequence that is necessary for post-transcriptional control ofrpsBexpression. The data allow one to infer the amino acid sequences of S2 and EF-Ts.