Direct DNA Sequencing of Complementary DNA Amplified by the Polymerase Chain Reaction
Direct DNA Sequencing of Complementary DNA Amplified by the Polymerase Chain Reaction
复制标题
聚合酶链式反应扩增的互补 DNA 的直接 DNA 测序
DOI：
10.1385/0-89603-205-1:9
复制
发表时间：
1991
影响因子：
2.6
通讯作者：
C. T. Caskey
中科院分区：
文献类型：
--
作者：
Richard A. Gibbs;P. Nguyen;C. T. Caskey
Protocols for the sequence analysis of conventional single-stranded or double-stranded DNA templates are often unsuitable for the direct sequencing of DNA fragments generated by the polymerase chain reaction (PCR) (1,2). The features that can distinguish PCR products as templates for sequencing include (a) contamination of the reactions by nonspecific PCR amplification products that are complementary to the sequencing primer, (b) the persistence of “leftover” PCR primers from the amplification reactions, and (c) the potential for competition between one strand of the amplified fragment and the oligonucleotide used for the sequencing. The various approaches that have been used to overcome these problems include
DOI：
10.1073/pnas.86.6.1919
发表时间：
1989-03-01
影响因子：
11.1
作者：
GIBBS, RA;NGUYEN, PN;CASKEY, CT
通讯作者：
CASKEY, CT
通过对致癌物诱发肿瘤的酶促扩增 DNA 进行直接测序来表征 c-Ki-ras 致癌基因等位基因。
DOI：
10.1073/pnas.84.14.4974
发表时间：
1987
影响因子：
11.1
作者：
McMahon,G;Davis,E;Wogan,GN
通讯作者：
Wogan,GN