Highly Sensitive Amperometric Cholesterol Biosensor Based on Pt-Incorporated Fullerene-like ZnO Nanospheres
Highly Sensitive Amperometric Cholesterol Biosensor Based on Pt-Incorporated Fullerene-like ZnO Nanospheres
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DOI：
10.1021/jp9089497
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发表时间：
2010-01-14
影响因子：
3.7
通讯作者：
Zhu, Jing
中科院分区：
文献类型：
--
作者：
Ahmad, Mashkoor;Pan, Caofeng;Zhu, Jing
研究了一种基于pt掺杂的类富勒烯ZnO杂化纳米球的高灵敏度安培生物传感器。在玻璃碳电极（GCE）上成功制备了直径在50 ~ 200 nm之间的Pt-ZnO纳米球（PtZONS）。采用高分辨率透射电镜（HRTEM）和能谱仪（EDS）对ZnO纳米球中的Pt纳米粒子进行了表征。掺杂的Pt纳米颗粒表现出电催化过氧化氢氧化的能力，并显著提高了响应电流。PtZONS/GCE对过氧化氢的灵敏度为147.8 μ A μ M-1 cm(-2)，远高于传统电极。用胆固醇氧化酶（ChOx）对PtZONS/GCE进行物理吸附功能化。酶电极对胆固醇的灵敏度为1886.4 mA M-1 cm(-2)，响应时间小于5 s，线性范围为0.5 ~ 15 μ m。此外，研究表明，该生物传感器具有良好的抗干扰能力，并且在相对较长的储存时间（超过5周）内具有良好的稳定性。这些结果表明，PtZONS不仅提高了对胆固醇的敏感性，而且有助于消除低施加电位下的干扰。
A highly sensitive amperometric biosensor based on Pt-incorporated fullerene-like ZnO hybrid nanospheres has been investigated. Pt-ZnO nanospheres (PtZONS) with diameters in the range 50-200 nm have been successfully synthesized by electrodeposition on a glassy carbon electrode (GCE). The Pt nanoparticles in ZnO nanospheres have been identified with high-resolution transmission electron microscopy (HRTEM) and energy dispersive X-ray spectroscopy (EDS). The doped Pt nanoparticles demonstrate the abilities to electrocatalyze the oxidation of hydrogen peroxide and substantially raise the response current. The sensitivity of the PtZONS/GCE to hydrogen peroxide is 147.8 mu A mu M-1 cm(-2), which is much higher than that of a conventional electrode. The PtZONS/GCE was functionalized with cholesterol oxidase (ChOx) by physical adsorption. The enzyme electrode exhibits a very high and reproducible sensitivity of 1886.4 mA M-1 cm(-2) to cholesterol with a response time less than 5 s and a linear range from 0.5 to 15 mu M. Furthermore, it has been revealed that the biosensor exhibits a good anti-interference ability and favorable stability over relatively long-term storage (more than 5 weeks). All these results strongly suggest that the PtZONS not only enhance the sensitivity to cholesterol but also help to eliminate the interference at low applied potential.