INTRICATE COMBINATORIAL PATTERNS OF EXON SPLICING GENERATE MULTIPLE REGULATED TROPONIN-T ISOFORMS FROM A SINGLE GENE
INTRICATE COMBINATORIAL PATTERNS OF EXON SPLICING GENERATE MULTIPLE REGULATED TROPONIN-T ISOFORMS FROM A SINGLE GENE
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DOI：
10.1016/0092-8674(85)90062-5
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
NADALGINARD, B
中科院分区：
文献类型：
--
作者：
BREITBART, RE;NGUYEN, HT;NADALGINARD, B
选择性RNA剪接的机制，在蛋白质多样性的产生中很重要，是常见的，但不完全了解。在收缩蛋白中，肌钙蛋白T存在于几种同种异构体中，部分来源于大鼠骨骼快速肌钙蛋白T基因3‘’区差异RNA剪接的新模式。事实上，这个基因是一种以前未曾预料到的编码多种同种异构体的能力。从该基因中分离出4个不同但相关的c[互补]DNA，它们在其5”区域共享序列身份的不连续亚段，并确定了基因组序列，表明具有特征分裂密码子结构的小外显子以复杂的组合模式差异剪接，产生至少10个，可能64个不同的肌钙蛋白T mRNA，编码不同的同种异构体。以发育调节和组织特异性的方式。这些mRNA中至少有2个是由结构相同的初级转录物剪接而来，需要通过反式作用因子进行控制。
Mechanisms of alternative RNA splicing, important in the generation of protein diversity, are common but incompletely understood. Among the contractile proteins, troponin T exists in several isoforms, shown to be derived in part from a novel pattern of differential RNA splicing in the 3'' region of the rat skeletal fast troponin T gene. In fact, this gene was a previously unsuspected capacity to encode multiple isoforms. The isolation of 4 distinct but related c[complementary]DNA from this gene, which share discontinuous subsegments of sequence identity in their 5'' regions, and the determination of the genomic sequence, demonstrate that small exons with characteristic split codon structure are differentially spliced in intricate combinatorial patterns to generate a minimum of 10, and potentially 64, distinct troponin T mRNA, encoding different isoforms, in a developmentally regulated and tissue-specific manner. At least 2 of these mRNA are spliced from structurally identical primary transcripts, necessitating control by trans-acting factors.