CLONAL ANALYSIS OF VERTEBRATE MYOGENESIS .8. FIBROBLAST GROWTH-FACTOR (FGF) DEPENDENT AND FGF-INDEPENDENT MUSCLE COLONY TYPES DURING CHICK WING DEVELOPMENT
CLONAL ANALYSIS OF VERTEBRATE MYOGENESIS .8. FIBROBLAST GROWTH-FACTOR (FGF) DEPENDENT AND FGF-INDEPENDENT MUSCLE COLONY TYPES DURING CHICK WING DEVELOPMENT
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DOI：
10.1016/0012-1606(88)90264-3
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发表时间：
1988-07-01
影响因子：
2.7
通讯作者：
HAUSCHKA, SD
中科院分区：
文献类型：
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作者：
SEED, J;HAUSCHKA, SD
研究了牛成纤维细胞生长因子（FGF）对鸡翅膀发育过程中不同阶段骨骼肌集落形成（MCF）细胞群体外分化的影响。结果表明，牛FGF（每天3 mg/ml）使从第4-12天翅芽中获得的MCF细胞的分化开始延迟约1天;但是，此外，结果表明，来自第23-27期（第4-5天）胚胎的集落形成细胞亚群需要FGF进行肌源性分化。FGF依赖性MCF细胞在没有FGF的情况下附着并生长，但除非在接种后1-3天内给予FGF，否则不分化。因此，在阶段23和27之间，肌原性群体包含FGF依赖性的离散亚类和FGF非依赖性的其它亚类。这两个亚类都存在于先前分类的两种MCF细胞群中，即早期和晚期MCF细胞。FGF依赖性和独立的早期MCF细胞存在于翅芽中，直到第25阶段，之后只有FGF独立的早期MCF亚类持续存在。类似地，FGF依赖性和非依赖性晚期MCF细胞都存在于第25期和第27期之间，但在第31期之后仅保留FGF非依赖性晚期MCF亚类。负责MCF细胞亚类比例的相对变化和FGF需求的机制尚不清楚。此外，虽然需要FGF，但没有证据表明FGF触发FGF依赖性MCF细胞亚类内的骨骼肌终末分化。
The effect of bovine fibroblast growth factor (FGF) on the in vitro differentiation of various stage-specific populations of skeletal muscle colony-forming (MCF) cells from the developing chick wing bud was examined. The results show that bovine FGF (3 mg/ml daily) delays the onset of differentiation of MCF cells obtained from Day 4-12 wing buds by about 1 day; but, in addition, the results demonstrate that a subset of colony-forming cells derived from stage 23-27 (Day 4-5) embryos require FGF for myogenic differentiation. The FGF-dependent MCF cells attach and grow in the absence of FGF, but do not differentiate unless given FGF within 1-3 days after inoculation. Thus, between stages 23 and 27 the myogenic population contains discrete subclasses that are FGF dependent and others that are FGF independent. Both sublcasses are found within two of the previously classified MCF cell populations, the early and late MCF cells. FGF-dependent and independent early MCF cells are present within the wing bud until stage 25, after which only the FGF-independent early MCF subclass persists. Similarly, both FGF-dependent and -independent late MCF cells are present between stages 25 and 27, but only the FGF-independent late MCF subclass remains after stage 31. The mechanisms responsible for relative changes in the proportions of MCF cell subclasses and for the FGF requirements are not understood. In addition, while FGF is required, there is no evidence suggesting that FGF triggers skeletal muscle terminal differentiation within the FGF-dependent MCF cell subclasses.