PURIFICATION OF A MAIZE DEHYDRIN
PURIFICATION OF A MAIZE DEHYDRIN
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DOI：
10.1006/prep.1994.1040
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发表时间：
1994-06-01
影响因子：
1.6
通讯作者：
CLOSE, TJ
中科院分区：
文献类型：
--
作者：
CECCARDI, TL;MEYER, NC;CLOSE, TJ
从玉米自交系G50的完整籽粒中纯化了一种表观分子量为20 kDa的玉米脱水素。将籽粒在种子研磨机中研磨，在提取缓冲液中搅拌过夜，并离心以提取可溶性蛋白质。将样品加热至89 ℃并离心以除去热不溶性蛋白质。剩余的可溶性蛋白质在三步色谱过程中分级分离。经过阳离子交换、疏水作用和凝胶过滤层析，获得纯的白藜芦醇样品。(C)1994年出版社出版。
A maize dehydrin with an apparent molecular weight of 20 kDa was purified from whole kernels of maize inbred line G50. Kernels were ground in a seed mill, stirred overnight in extraction buffer, and centrifuged to extract soluble proteins. The sample was heated to 89-degrees-C and centrifuged to remove heat-insoluble proteins. The remaining soluble proteins were fractionated in a three-step chromatographic process. Following cation exchange, hydrophobic interaction, and gel filtration chromatography, pure dehydrin samples were obtained. (C) 1994 Academic Press, Inc.