RAMAN-SPECTROSCOPY OF NORMAL AND DISEASED HUMAN BREAST TISSUES
RAMAN-SPECTROSCOPY OF NORMAL AND DISEASED HUMAN BREAST TISSUES
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DOI：
10.1021/ac00101a001
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发表时间：
1995-03-01
影响因子：
7.4
通讯作者：
REDD, DCB
中科院分区：
文献类型：
--
作者：
FRANK, CJ;MCCREERY, RL;REDD, DCB
将组织学正常的人乳腺活检样品的拉曼光谱与表现出浸润性导管癌（IDC）或纤维囊性变化的那些样品进行比较。实验在784 nm的CCD检测器减少荧光干扰，并产生高信噪比光谱与相对较低的激光功率（10-200 mW）。对于主要拉曼谱带的比率，正常标本的样品与样品和患者与患者的变化小于5%。患病标本的拉曼光谱发生了显著变化，脂质谱带明显变弱。浸润性导管癌样品的光谱与人胶原蛋白的光谱相似。良性（纤维囊性）和恶性（IDC）病变之间的差异小于正常和IDC标本之间的差异，但仍然是可重复的。光纤采样通过皮下注射针头和远程探头进行了演示。考虑了用拉曼光谱进行快速诊断的可能性。
Raman spectra of histologically normal human breast biopsy samples were compared to those exhibiting infiltrating ductal carcinoma (IDC) or fibrocystic change. Experiments at 784 mn with CCD detectors reduced fluorescence interference and produced high SNR spectra with relatively low (10-200 mW) laser power. Sample to sample and patient to patient variation for normal specimens were less than 5% for the ratios of major Raman bands. The Raman spectra changed dramatically in diseased specimens, with much weaker lipid bands being evident. The spectrum of infiltrating ductal carcinoma samples is similar to that of human collagen. Differences between benign (fibrocystic) and malignant (IDC) lesions were smaller than those between normal and IDC specimens, but were still reproducible. Fiberoptic sampling through a hypodermic needle and with a remote probe were demonstrated. The possibility of rapid diagnosis with Raman spectroscopy is considered.