Expression of Hes6 and NeuroD in the olfactory epithelium, vomeronasal organ and non-sensory patches.
Expression of Hes6 and NeuroD in the olfactory epithelium, vomeronasal organ and non-sensory patches.
复制标题
Hes6 和 NeuroD 在嗅上皮、犁鼻器和非感觉斑中的表达。
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
N. Obara
中科院分区：
文献类型：
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作者：
Yuko Suzuki;I. Mizoguchi;Hiromasa Nishiyama;M. Takeda;N. Obara
碱性螺旋-环-螺旋转录因子NeuD和Hes6促进神经元分化。观察它们在小鼠后鼻腔嗅觉上皮(OE)、犁鼻器(VNO)和无感觉斑块中的表达。原位杂交检测到Hes6在胚胎OE的基底层表达。出生后，Hes6在基底膜上方的球状基底层细胞(GBCS)中有表达。神经D在胚胎OE中的表达与前述一致，而在出生后的OE中，在GBC层细胞和GBCS上层细胞中均可检测到。在迷走神经感觉上皮(S-迷走神经感觉上皮)中，Hes6在胚胎第12天开始表达，之后仅局限于S感觉迷走神经基底区域的单层细胞，在该区域存在表达Hes5的未分化细胞。在胚胎期，NeuroD在整个S-迷走神经核中均有表达。生后，在S-迷走神经核与非感觉性迷走神经核交界处可见Hes6和Neurod的表达。免疫组织化学染色显示，直至P3，神经D阳性细胞仍存在于S室旁核的边缘和中央。这些结果表明，Hes6和NeuroD在化学感受器神经元的前体细胞中都有表达，并且Hes6的表达先于NeuroD的表达。此外，在去球小鼠再生的VNO中，在S-VNO的边缘和中心都观察到了NeuroD阳性细胞，表明这两个区域都发生了神经元翻转。此外，在后鼻腔的背窝，由于Orns和GBCs的程序性死亡，在出生后10天到21天之间形成了几个无感觉斑块。在胚胎期，Hes6和NeuD在OE中的表达没有地区差异。在P3-P7，在推测的非感觉性斑块对应的区域，Neurod和Hes6的表达消失。这些基因的缺失可能会阻止GBCS和Orns的分化，并可能导致其凋亡。
Basic helix-loop-helix transcription factors NeuroD and Hes6 promote neuronal differentiation. The expression of their genes in the olfactory epithelium (OE), vomeronasal organ (VNO) and the non-sensory patches of the posterior nasal cavity of mice was examined. As detected by in situ hybridization, Hes6 was expressed in a basal progenitor layer of the embryonic OE. After birth, the expression of Hes6 was detected in a cell layer above the basement membrane, globose basal cells (GBCs). Expression of NeuroD in the embryonic OE was in agreement with that previously described; and in the postnatal OE, it was detected in cells of GBC layer and cells upper to GBCs. In the VNO, Hes6 was expressed throughout the sensory epithelium (S-VNO) at embryonic day 12, and later became restricted to a single layer of cells in the basal region of the S-VNO, where Hes5-expressing undifferentiated cells were present. NeuroD was expressed throughout the S-VNO during the embryonic stage. After birth, Hes6 and NeuroD expressions were observed in the border between the S-VNO and non-sensory VNO. Immunohistochemistry using anti-NeuroD antibody revealed that NeuroD-positive cells were still present not only at the edges but also in the center of the S-VNO until P3. These findings suggest that Hes6 and NeuroD are expressed in progenitors of chemoreceptor neurons and that the expression of Hes6 precedes that of NEUROD: Moreover, in the regenerating VNO of bulbectomized mice, NeuroD-positive cells were observed both at the edges and in the center of the S-VNO, suggesting that neuronal turnover occurred in both regions. Moreover, in the dorsal fossa of the posterior nasal cavity, several non-sensory patches are formed between postnatal (P) days 10 and 21 because of programmed death of ORNs and GBCs. During embryonic stages, the expression of Hes6 and NeuroD in the OE showed no regional differences. At P3-P7, expression of NeuroD and Hes6 disappeared in the region corresponding to the presumptive non-sensory patches. The loss of these genes may stop the differentiation and may cause apoptosis of GBCs and ORNs.
影响因子：
2.7
作者：
Olson, JM;Asakura, A;Tapscott, SJ
通讯作者：
Tapscott, SJ
影响因子：
10.5
作者：
Naya, FJ;Huang, HP;Tsai, MJ
通讯作者：
Tsai, MJ