Oocyte Penetration and Acrosome Reactions of Human Spermatozoa I: Influence of Incubation Time and Medium Composition
Oocyte Penetration and Acrosome Reactions of Human Spermatozoa I: Influence of Incubation Time and Medium Composition
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人类精子的卵母细胞穿透和顶体反应 I：孵育时间和培养基成分的影响
DOI：
10.1111/j.1439-0272.1986.tb01753.x
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发表时间：
2009
期刊：
影响因子：
2.4
通讯作者：
A. Campana
中科院分区：
文献类型：
--
作者：
R. J. Kooij;M. Balerna;A. Roatti;A. Campana
总结：评价了洗涤后的精子悬液穿透无精子仓鼠卵母细胞的能力和体外表现出顶体反应的能力。 发现顶体反应的百分比（AR%）随着孵育时间和牛血清白蛋白（BSA）浓度的增加而增加。然而，我们还发现，在从一些有生育能力的男性获得的精子样本中，在长时间孵育期间，存活的、未反应的精子数量非常高。培养22小时后，用10%人脐带血清代替3.0%BSA培养的精子活力较高。仓鼠卵母细胞中的穿透率在10%血清或3.0% BSA作为培养基补充的情况下无显著差异。添加人血清白蛋白而不是牛血清白蛋白对精子的卵母细胞穿透率没有明显影响。
Summary: Washed sperm suspensions were evaluated for their ability to penetrate zona‐free hamster oocytes and to exhibit an acrosome reaction in vitro. The percentage of acrosome reactions (AR%) was found to increase with incubation time and increased bovine serum albumin (BSA) concentration. We also found, however, that a remarkably high number of live, unreacted spermatozoa persisted during prolonged incubation in sperm samples obtained from some fertile men. After 22 hrs incubation, the motility of the spermatozoa was higher when the medium was supplemented with 10% human cord serum instead of 3.0% BSA. Penetration rates in hamster oocytes were not significantly different with 10% serum or 3.0% BSA as medium‐supplementation. The addition of human instead of bovine serum albumin did not apparently affect the oocyte penetration rate of the spermatozoa.