Culture conditions and the development of the photosynthetic mechanism. II. An apparatus for the continuous culture of Chlorella
Culture conditions and the development of the photosynthetic mechanism. II. An apparatus for the continuous culture of Chlorella
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DOI：
10.1085/jgp.28.2.103
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发表时间：
1944-01-01
期刊：
影响因子：
--
通讯作者：
Clark, L. B.
中科院分区：
文献类型：
--
作者：
Myers, Jack;Clark, L. B.
描述了一种用于连续培养单细胞藻类的装置。藻类悬浮液包含在2个玻璃管之间的5 mm环中。通过自动稀释悬浮液的光度计装置获得恒定密度的悬浮液，使得培养物连续保持在生长曲线上的一个点。在任何所需的时间间隔收获部分悬浮液，留下部分作为下一个间隔的接种物。在一个培养物上生长3周的数据表明，藻类的生理特性是恒定的，并且日复一日地可重复。该装置提供了一种exptl的来源。高均匀性的材料和/或稳定内部变量的手段，以便可以探索培养条件与光合作用行为的关系。|| 摘要评论：杰克·迈尔斯
An apparatus is described for continuous culture of unicellular algae. The algal suspension is contained in a 5-mm. annulus between 2 glass tubes. Constant density of suspension is obtained by a photometric device which automatically dilutes the suspension so that a culture is maintained continuously at one point on the growth curve. Part of the suspension is harvested at any desired time interval, leaving part as an inoculum for the next interval. Data presented on one culture grown over a period of 3 weeks indicate that the physiol. characteristics of the algae are constant and reproducible day after day. The apparatus provides a source of exptl. material of high uniformity and/or a means of stabilizing internal variables so that the relation of culture conditions to photosynthetic behavior may be explored. || ABSTRACT AUTHORS: Jack Myers