Image-guided transplantation of single cells in the bone marrow of live animals.
Image-guided transplantation of single cells in the bone marrow of live animals.
复制标题
活体动物骨髓中单细胞的图像引导移植。
DOI：
10.1038/s41598-017-02896-6
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发表时间：
2017
影响因子：
4.6
通讯作者：
Lin,CharlesP
中科院分区：
文献类型：
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作者：
Turcotte,Raphaël;Alt,Clemens;Runnels,JudithM;Ito,Kyoko;Wu,JuwellW;Zaher,Walid;Mortensen,LukeJ;Silberstein,Lev;Côté,DanielC;Kung,AndrewL;Ito,Keisuke;Lin,CharlesP
单个造血干细胞的移植是其功能表征的重要方法，但标准移植方案依赖于静脉注射后细胞归巢到骨髓。在这里，我们提出了一种将单细胞直接移植到活小鼠骨髓中的方法。我们开发了一个光学平台，它集成了一个多光子显微镜与激光消融单元的显微手术和细胞显微操作的光镊。这些工具允许具有高度空间控制的图像引导的单细胞移植。该平台用于递送单个造血干细胞。随着时间的推移，移植物的植入被跟踪，说明该技术可用于研究体内正常和恶性干细胞。
Transplantation of a single hematopoietic stem cell is an important method for its functional characterization, but the standard transplantation protocol relies on cell homing to the bone marrow after intravenous injection. Here, we present a method to transplant single cells directly into the bone marrow of live mice. We developed an optical platform that integrates a multiphoton microscope with a laser ablation unit for microsurgery and an optical tweezer for cell micromanipulation. These tools allow image-guided single cell transplantation with high spatial control. The platform was used to deliver single hematopoietic stem cells. The engraftment of transplants was tracked over time, illustrating that the technique can be useful for studying both normal and malignant stem cellsin vivo.