Prevalence, Bacterial Load, and Antimicrobial Resistance of Salmonella Serovars Isolated From Retail Meat and Meat Products in China
Prevalence, Bacterial Load, and Antimicrobial Resistance of Salmonella Serovars Isolated From Retail Meat and Meat Products in China
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中国零售肉类和肉制品中分离出的沙门氏菌血清型的流行率、细菌载量和耐药性
DOI：
10.3389/fmicb.2019.02121
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发表时间：
2019-09
影响因子：
5.2
通讯作者：
Wei Xianhu
中科院分区：
文献类型：
--
作者：
Yang Xiaojuan;Wu Qingping;Zhang Jumei;Huang Jiahui;Chen Ling;Wu Shi;Zeng Haiyan;Wang Juan;Chen Moutong;Wu Haoming;Gu Qihui;Wei Xianhu
沙门氏菌仍然是中国报告的细菌性食源性疾病的主要原因。肉制品被认为是人类沙门氏菌病的主要来源之一；然而，缺乏关于这些食品受沙门氏菌污染的全面、定量的数据。因此，本研究的目的是调查中国地区零售肉类中不同沙门氏菌血清型的流行率、细菌载量和耐药性。2011年7月至2016年6月，共采集零售肉类样品807份，覆盖中国大部分省会城市。总体而言，159个样本(19.7%)对沙门氏菌检测呈阳性。污染率最高的是猪肉(37.3%，n=287)，其次是牛肉(16.1%，n=161)、羊肉(10.9%，n=92)、饺子(6.6%，n=212)和熏猪肉(3.6%，n=55)。最大可能数(MPN)分析表明，该方法阳性的样品(n=83)中，污染主要在0.3~10MPN/g之间，超过110MPN/g的样品有8个。共鉴定出29个血清型和33个多位点序列分型模式，其中以德比沙门氏菌、鼠伤寒沙门氏菌、伦敦沙门氏菌、里森沙门氏菌、S1、4、[5]、12：I：-、韦氏沙门氏菌和肠炎沙门氏菌最为常见。在218株非重复分离株中，181株(83.0%)对至少一种抗菌药耐药，128株(58.7%)对至少三种抗菌药耐药。耐药率较高的有四环素(65.6%)、氨苄西林(45.4%)、甲氧苄氨嘧啶-磺胺甲恶唑(40.8%)、链霉素(40.4%)和奈立地酸(35.8%)，除韦氏葡萄球菌外，其余7个血清型的耐药率和多重耐药率均高于优势较低的血清型。值得注意的是，所有印第安纳葡萄球菌对超广谱头孢菌素(包括头孢曲松和头孢吡肟)、环丙沙星和多种其他类别的抗生素都表现出耐药性。此外，两株S.1，4，[5]，12：I：-菌株对亚胺培南表现出耐药性。本研究对中国地区肉制品中分离的各种沙门氏菌的流行情况及耐药性进行了系统、全面的调查，揭示了其对公众健康的潜在危害。
Salmonella remains the leading cause of reported bacterial foodborne disease in China. Meat products are recognized as one of the major sources of human salmonellosis; however, there is a lack of comprehensive, quantitative data concerning Salmonella contamination of these foods. Therefore, the objectives of this study were to investigate the prevalence, bacterial load, and antimicrobial resistance profiles of various Salmonella serovars in retail meat across the whole of China. Between July 2011 and June 2016, a total of 807 retail meat samples were collected, covering most provincial capitals in China. Overall, 159 (19.7%) samples tested positive for Salmonella. The highest contamination rate occurred in pork (37.3%, n = 287), followed by beef (16.1%, n = 161), mutton (10.9%, n = 92), dumplings (6.6%, n = 212), and smoked pork (3.6%, n = 55). Most probable number (MPN) analysis revealed that contamination was mainly in the range of 0.3–10 MPN/g among those samples testing positive using this method (n = 83), with eight samples exceeding 110 MPN/g. Among the 456 Salmonella enterica subsp. enterica isolates obtained in this study, 29 serovars and 33 multilocus sequence typing patterns were identified, with S. Derby, S. Typhimurium, S. London, S. Rissen, S. 1,4,[5],12:i:-, S. Weltevreden, and S. Enteritidis being the most prevalent. Among the 218 non-duplicate isolates, 181 (83.0%) were resistant to at least one class of antimicrobials and 128 (58.7%) were resistant to at least three classes. High rates of resistance were observed for tetracycline (65.6%), ampicillin (45.4%), trimethoprim-sulfamethoxazole (40.8%), streptomycin (40.4%), and nalidixic acid (35.8%), with the seven most prevalent serovars, except S. Weltevreden, showing higher rates of resistance and multidrug resistance compared with the less dominant serovars. Of note, all S. Indiana isolates exhibited resistance to extended-spectrum cephalosporins (including ceftriaxone and cefepime), ciprofloxacin, and multiple other classes of antibiotics. Further, two S. 1,4,[5],12:i:- isolates showed resistance to imipenem. This study provides systematic and comprehensive data on the prevalence and antimicrobial resistance profiles of various Salmonella serovars isolated from meat products in China, indicating their potential risk to public health.
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DOI：
10.1016/j.ijmm.2013.06.003
发表时间：
2013
期刊：
International journal of medical microbiology : IJMM
影响因子：
--
作者：
W. Witte
通讯作者：
W. Witte
影响因子：
2.8
作者：
Lai, Jing;Wang, Yang;Wu, Congming
通讯作者：
Wu, Congming
影响因子：
5.3
作者：
Aslam, Mueen;Checkley, Sylvia;Boerlin, Patrick
通讯作者：
Boerlin, Patrick
中国武汉婴幼儿肠道沙门氏菌分离株的特征
影响因子：
5.2
作者：
Cui, Shenghui;Li, Jingyun;Ma, Yue
通讯作者：
Ma, Yue
影响因子：
2.8
作者：
Hendriksen, Rene S.;Vieira, Antonio R.;Aarestrup, Frank M.
通讯作者：
Aarestrup, Frank M.