Laser vascular welding--how does it work?
Laser vascular welding--how does it work?
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DOI：
10.1016/s0890-5096(06)60732-2
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发表时间：
1987-05
影响因子：
1.5
通讯作者：
Rodney A. White;G. Kopchok;Shi-Kaung Peng;R. Fujitani;Geoffrey H. White;Stanley R. Klein;Jouni Uitto
中科院分区：
文献类型：
--
作者：
Rodney A. White;G. Kopchok;Shi-Kaung Peng;R. Fujitani;Geoffrey H. White;Stanley R. Klein;Jouni Uitto
本研究对4个犬股动脉和股静脉形成的长2 cm的静脉和动静脉吻合的标本进行了组织学和电子显微镜观察。在将最高温度限制在44.2±1.6℃(平均值±SD)的过程中，持续用生理盐水冲洗焊缝，并在融合后立即取出标本并保存以供检查。组织学和电子显微镜检查显示了不同的融合机制，包括a)变性的胶原和弹性蛋白附着在中膜和外膜；b)中膜胶原和弹性蛋白结合到动脉的内部弹性膜上；c)融合由凝结的血小板和纤维蛋白组成，取决于血管边缘的对接和对接。这项研究表明，用Ar激光进行血管组织融合是通过多种机制进行的。未来的实验应该描绘出哪种类型的封闭物在融合时产生最佳的强度，并增强长期愈合。
This study evaluated the histology and electron microscopy of four samples of 2 cm long venotomles and artery-vein anastomoses formed in canine femoral arteries and veins using the argon laser (0.5 W power, 1 800 J/cm2, 4 min exposure/1 cm length of anastomosis). Welds were continuously irrigated with saline during the procedure to limit maximal temperatures to 44.2±1.6°C (mean±SD), and the specimens were removed immediately following fusion and preserved for examination. Histologic and electron microscopic examination of different areas of the welds revealed various mechanisms of fusion including a) apposition of denatured collagen and elastin in the media and adventitia; b) bonding of veln medial collagen and elastin to the internal elastic membrane of the artery; and c) fusion consisting of a coagulum of platelets and fibrin depending on the allgnment and apposition of the vessel edges. This study demonstrates that vascular tissue fusion by the argon laser occurs by various mechanisms. Future experiments should delineate which types of seal produce the optimal strength at the time of fusion, and enhance longterm healing.