Comparison of blood pro/antioxidant levels before and after acute exercise in athletes and non-athletes
Comparison of blood pro/antioxidant levels before and after acute exercise in athletes and non-athletes
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DOI：
10.4149/gpb_2012_025
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发表时间：
2012-06-01
影响因子：
1.5
通讯作者：
Jakovljevic, Vladimir Lj
中科院分区：
文献类型：
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作者：
Djordjevic, Dusica Z.;Cubrilo, Dejan G.;Jakovljevic, Vladimir Lj
我们的研究的目的是评估青少年运动员和非运动员在休息和急性暴露于体力负荷后的氧化还原状态，并找到氧化还原状态参数和受试者的形态功能特征之间的关系。对58名青少年手球运动员和37名非运动员进行了体成分分析、最大耗氧量测定和最大递增负荷运动试验前后即刻采血。在休息时，运动员有显着较高的超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性，较高的还原型谷胱甘肽（GSH）和一氧化氮（NO）水平和较低的脂质过氧化反应（TBARS）水平相比，非运动员。极量运动试验引起非运动员超氧阴离子自由基（O-2（-））、过氧化氢（H2 O2）和NO水平显著升高，而TBARS水平降低。运动员经历了NO水平的下降和CAT活性的下降。运动后，运动员的O-2（-）水平显著低于非运动员。双因素重复测量方差分析表明，O-2（-），NO和TBARS对运动试验的反应依赖于受试者的运动参与（训练经验）。形态功能参数与氧化还原参数之间存在显著相关性，表明体能影响氧化还原稳态。
The aims of our study were to assess the redox state of adolescent athletes and non-athletes both at rest and after acute exposure to physical load and to find relations between parameters of redox state and morphofunctional characteristics of subjects. 58 young handball players and 37 non-athletes were subjected to body composition analysis, measuring of maximal oxygen consumption and blood sampling immediately before and after a maximal progressive exercise test. At rest, athletes had significantly higher superoxide dismutase (SOD) and catalase (CAT) activity, higher levels of reduced glutathione (GSH) and nitric oxide (NO) and lower levels of lipid peroxidation (TBARS) compared with non-athletes. A maximal exercise test induced statistically significant rise of superoxide anion radical (O-2(-)), hydrogen peroxide (H2O2) and NO levels in non-athletes, while TBARS levels decreased. Athletes experienced the fall in NO levels and the fall in CAT activity. After exercise, athletes had significantly lower levels of O-2(-) compared with non-athletes. Two way repeated measures ANOVA showed that the response of O-2(-), NO and TBARS to the exercise test was dependent on the sports engagement (training experience) of subjects. Significant correlations between morphofunctional and redox parameters were found. These results suggest that physical fitness affects redox homeostasis.