Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
复制标题
在自养和混合营养条件下两种莱茵衣藻（草藻目、绿藻纲）菌株对亚致死铜暴露的反应
DOI：
10.2216/09-101.1
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发表时间：
2011
期刊：
影响因子：
1.6
通讯作者：
M. C. Rodríguez
中科院分区：
文献类型：
--
作者：
S. Sabatini;P. Leonardi;M. C. Rodríguez
Sabatini S.E.，Leonardi P.I.和Rodríguez M.C. 2011.响应亚致死铜暴露在两个菌株的莱茵衣藻（团藻目，绿藻）在自养和兼养条件。Phycologia 50：78-88. DOI：10.2216/09-101.1本工作的目的是确定两种莱茵衣藻菌株对亚致死铜暴露（20和40 µM CuCl 2）的反应：野生型4A+和多效性突变体cur 9，最初被选为混合营养条件下的耐铜表型。在低辐射（50 µmol m−2 s−1）下，在自养和兼养培养基中进行生物测定。在开始时，通过MINEQL软件估计培养基中的Cu 2+浓度。氧化应激指标，如光合色素比率，过氧化氢酶活性，硫代巴比妥酸反应物质（TBARS）和生长速率的浓度的变化，表现出更好的性能的野生型和突变株在自养和兼养条件下，分别。中度氧化胁迫伴随着超微结构的变化，如淀粉和空泡的积累与电子致密的存款和膜结构，以及轻度类囊体和线粒体嵴解体。在混合营养条件下，异柠檬酸裂解酶（ICL）的活性在孵育72小时后达到峰值。验证了过氧化氢酶（CAT）和ICL活性之间的反比关系，并证明是一个很好的指标，氧化应激相关的其他特征，如抑郁症的生长速度或TBARS剂量的增加一致。
Sabatini S.E., Leonardi P.I. and Rodríguez M.C. 2011. Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions. Phycologia 50: 78–88. DOI: 10.2216/09-101.1 The aim of this work was to determine the responses to sublethal copper exposure (20 and 40 µM CuCl2) in two Chlamydomonas reinhardtii strains: the wild type 4A+ and the pleiotropic mutant cur9, originally selected as a copper-tolerant phenotype in mixotrophic conditions. Bioassays were performed in both autotrophic and mixotrophic media, under low irradiation (50 µmol m−2 s−1). At the onset, Cu2+ concentration in culture media was estimated by MINEQL software. Oxidative stress indicators, such as changes in photosynthetic pigment ratios, catalase activity, concentration of thiobarbituric acid reactive substances (TBARS) and growth rate, demonstrated a better performance of the wild type and the mutant strain in autotrophic and mixotrophic conditions, respectively. Moderate oxidative stress was accompanied by ultrastructural changes, such as accumulation of starch and vacuoles with electronic-dense deposits and membranous structures together with mild thylakoidal and mitochondrial crest disorganization. In mixotrophic conditions isocitrate lyase (ICL) activity peaked after 72 h incubation. An inverse relation between catalase (CAT) and ICL activities was verified and proved to be a good indicator of oxidative stress correlating consistently with other characteristic features such as depression in growth rate or increase in TBARS dosage.