Cryopreservation of Human Pluripotent Stem Cell-Derived Cardiomyocytes: Strategies, Challenges, and Future Directions.
Cryopreservation of Human Pluripotent Stem Cell-Derived Cardiomyocytes: Strategies, Challenges, and Future Directions.
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人类多能干细胞来源的心肌细胞的冷冻保存：策略、挑战和未来方向。
DOI：
10.1007/978-3-319-45457-3_10
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发表时间：
2016
影响因子：
--
通讯作者：
Xu,Chunhui
中科院分区：
文献类型：
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作者：
Preininger,MarcelaK;Singh,Monalisa;Xu,Chunhui
近年来，人多能干细胞衍生的心肌细胞（human pluripotent stem cell-derived cardiomyocytes，hPSC-CMs）已成为遗传性心血管疾病体外模型、药物筛选和体内心脏再生研究的重要细胞来源。展望未来，在不损害其正常生化和生理功能的情况下有效冷冻保存hPSC-CM的能力将极大地促进其各种生物医学应用。虽然已经建立了用于冷冻、储存和解冻hPSC-CM的工作方案，但问题仍然是它们是否是最佳的。在这一章中，我们讨论了我们目前的理解属于hPSC-CMs的冷冻保存，并提供关键问题的机械，收缩，和再生特性的冷冻保存hPSC-CMs。
In recent years, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have emerged as a vital cell source forin vitromodeling of genetic cardiovascular disorders, drug screening, andin vivocardiac regeneration research. Looking forward, the ability to efficiently cryopreserve hPSC-CMs without compromising their normal biochemical and physiologic functions will dramatically facilitate their various biomedical applications. Although working protocols for freezing, storing, and thawing hPSC-CMs have been established, the question remains as to whether they are optimal. In this chapter, we discuss our current understanding of cryopreservation appertaining to hPSC-CMs, and proffer key questions regarding the mechanical, contractile, and regenerative properties of cryopreserved hPSC-CMs.