Procyanidins as antioxidants and tumor cell growth modulators
Procyanidins as antioxidants and tumor cell growth modulators
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DOI：
10.1021/jf0526487
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发表时间：
2006-03-22
影响因子：
6.1
通讯作者：
Mateus, N
中科院分区：
文献类型：
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作者：
Faria, A;Calhau, C;Mateus, N
对葡萄籽提取物进行分馏，得到了结构复杂程度不同的5个原花青素组分。研究了原花青素部分在脂质体膜系统中抑制2,2′-偶氮氮-2-甲基-丙氨酰胺二盐酸诱导的脂质过氧化的能力。通过监测氧消耗和测量共轭二烯的形成来评估所有馏分的抗氧化能力。所有测试的组分都通过增加氧化诱导时间来保护膜免受过氧自由基的侵害。这种效应在组分II前增加，但随着进一步原花青素组分结构复杂性的增加而降低，这可能是由于更复杂的组分表现出空间位阻效应。采用2,2 -二苯基-1-吡啶酰肼法和铁还原/抗氧化能力法分别测定了各组分的抗自由基性能和还原能力。此外，3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴还原和DNA合成在密歇根州癌症基金会7 (MCF-7)，人类乳腺癌细胞系，用儿茶素或原花青素部分处理，以评估这些化合物对细胞活力和增殖的影响。结果表明，在30 μ g/mL浓度下，组分1和组分11降低了细胞活力和增殖能力，而在60 μ g/mL浓度下则无此现象。儿茶素在30和60 μ g/mL浓度下也能降低细胞活力和增殖能力。有趣的是，显示出较高抗氧化活性的原花青素组分对细胞活力和增殖的影响是相同的。
Five procyanidin fractions with different structural complexities were obtained after fractionation of a grape seed extract. The procyanidin fraction's abilities to inhibit lipid peroxidation induced by 2,2'-azobis-2-methyl-propanimidamide dihydrochloride in a liposomal membrane system were examined. The antioxidant capacities of all fractions were evaluated through monitoring oxygen consumption and by measuring the formation of conjugated dienes. All tested fractions provided protection of membranes against peroxyl radicals by increasing the induction time of oxidation. This effect increased up to fraction II but decreased with the increase of the structural complexity of further procyanidin fractions, possibly due to steric hindrance effects exhibited by the more complex fractions. In addition, the antiradical properties and the reducing power of these fractions were determined by using 2,2diphenyl-1-picrylhydrazyl and ferric reducing/antioxiclant power methods, respectively. Moreover, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide reduction and DNA synthesis were measured in Michigan Cancer Foundation 7 (MCF-7), a human breast cancer cell line, treated with catechin or procyanidin fractions in order to evaluate the effect of these compounds on cell viability and proliferation. The results obtained showed that at 30 mu g/mL, fractions I and 11 decreased cell viability and proliferation, which was not observed with 60 mu g/mL of the same fractions. Catechin was also able to decrease cell viability and proliferation at 30 and 60 mu g/mL. It is interesting to notice that the procyaniclin fractions that exhibited higher antioxidant activity were the same to affect cell viability and proliferation.