Disentangling the multiple effects of a novel high pressure jet device upon bacterial cell disruption
Disentangling the multiple effects of a novel high pressure jet device upon bacterial cell disruption
复制标题
DOI：
10.1016/j.cej.2017.04.067
复制
发表时间：
2017-09
影响因子：
15.1
通讯作者：
Li Xie;A. Terada;M. Hosomi
中科院分区：
文献类型：
--
作者：
Li Xie;A. Terada;M. Hosomi
高压喷射装置（HPJD）支持高通量，并且在减少过量活性污泥方面具有成本效益。我们的目的是确定摩擦剪切，碰撞和空化对细菌细胞破裂和细胞间物质洗脱的HPJD之间的主导机制。修改了HPJD以分离这三种机制，以便可以单独分析。革兰氏阴性菌和革兰氏阳性菌（分别为大肠杆菌和枯草芽孢杆菌）均受到改良HPJD装置的处理。剪切、碰撞和空泡作用分别造成11.12 ± 1.12%、11.97 ± 1.12%和23.70 ± 2.35%的损伤。colicell分别为5.2 ± 1.57%、7.81 ± 1.09%和28.54 ± 3.45%。subtiliscell，分别。利用原子力显微镜（AFM）观察到了不同形态的E. coliandB.由摩擦剪切、碰撞和空化作用引起的枯草杆菌细胞。剪切和碰撞变形E。B.大肠杆菌，而非大肠杆菌。subtiliscells，只是增加细胞表面粗糙度。相比之下，空泡直接导致了B的内爆。枯草杆菌细胞内的DNA、多糖和蛋白质的释放浓度高于摩擦剪切和碰撞。尽管有不同的细胞破坏机制，空化通常提供了最强烈的影响，细胞解体和细胞内成分的释放。coliandB.枯草杆菌。
High-pressure jet devices (HPJDs) support high throughput and are cost-effective in reducing excess activated sludge. We aimed to identify the dominant mechanism among frictional shear, collision, and cavitation on bacterial cell disruption and intercellular substances eluted by an HPJD. An HPJD was modified to disassociate these three mechanisms so that they could be analyzed separately. Gram-negative and Gram-positive bacteria (Escherichia coliandBacillus subtilis, respectively) were subjected to treatment by the modified HPJD device. Frictional shear, collision, and cavitation were found to damage 11.12 ± 1.12%, 11.97 ± 1.12%, and 23.70 ± 2.35% ofE. colicell and 5.2 ± 1.57%, 7.81 ± 1.09%, and 28.54 ± 3.45% ofB. subtiliscell, respectively. The application of atomic force microscopy (AFM) displayed different morphological changes ofE. coliandB. subtiliscells induced by frictional shear, collision, and cavitation. Frictional shear and collision deformedE. colicells but not severelyB. subtiliscells, merely increasing the cell surface roughness. In contrast, cavitation directly contributed to implosion ofB. subtiliscells, resulting in higher concentrations of released intracellular DNA, polysaccharide and protein than those by frictional shear and collision. Despite the distinct cell disruption mechanisms, cavitation commonly provided the most intense effect on cell disintegration and the release of intracellular components for bothE. coliandB. subtilis.