Ovary Slice Culture of Lilium formosanum WALLACE
Ovary Slice Culture of Lilium formosanum WALLACE
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台湾百合 WALLACE 子房切片培养
DOI：
10.1270/jsbbs1951.36.304
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发表时间：
1986
期刊：
影响因子：
2.4
通讯作者：
Eui
中科院分区：
文献类型：
--
作者：
M. Hayashi;K. Kanoh;Y. Serizawa;Eui
采用百合子房离体培养新技术，获得了大量的试管苗。以台湾百合（LiliumformosanumWALLACE）自花授粉后20天的子房为材料，将子房切成2mm厚的圆片。接种于32种改良MS琼脂培养基上。这些培养基的排列是基于正交表的L32表，并且15种培养基元素（每个具有2个水平）彼此组合，包括pH、蔗糖、主要盐、次要盐、维生素和几种激素。接种后40或50天，胚珠生长良好并可识别为种子。它们在试管中相继发芽。较高的pH值（6.3）和蔗糖浓度（8%）处理效果较好，而IAA（1ppm）处理则抑制了种子的成熟。接种后170 d，主要盐的标准浓度优于较低浓度，而激素的抑制作用有下降的趋势。
A new technique of ovary culture was applied in Lilium, resulting in the formation of a lot number of plantlets in vitro. Ovaries of Lilium formosanum WALLACE, sampled 20 days after self-pollination, were sliced into disks 2mm thick. They were inoculated on 32 kinds of modified MS agar media. The arrangement of those media was based on the L 32 table of orthogonal arrays, and 15 medium elements with 2 levels each, including pH, sucrose, major salts, minor salts, vitamins and several hormones, were combined with one another. Ovules grew well and became recognizable as seeds 40 or 50 days after inoculation. They germinated successively in the test tubes. Higher pH value (6.3) and sucrose concentration (8%) gave better results than lower ones, and the use of IAA (1 ppm) inhibited seed maturation. Standard concentration of the major salts was preferable to a lower one, while the inhibitory effect of hormones tended to decrease 170 days after inoculation.