Immunogenicity and protective efficacy of an elastase-dependent live attenuated swine influenza virus vaccine administered intranasally in pigs
Immunogenicity and protective efficacy of an elastase-dependent live attenuated swine influenza virus vaccine administered intranasally in pigs
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DOI：
10.1016/j.vaccine.2010.08.003
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发表时间：
2010-10-08
期刊：
影响因子：
5.5
通讯作者：
Zhou, Yan
中科院分区：
文献类型：
--
作者：
Masic, Aleksandar;Lu, Xinya;Zhou, Yan
甲型流感病毒是一种重要的猪呼吸道病原体，对养猪业造成严重的经济影响。接种疫苗是预防和控制流感感染的首选。减毒活流感疫苗（LAIV）已被批准用于人和马，其应用提供了广泛的保护性免疫，但市场上没有针对猪流感病毒（SW）的LAIV。以前我们报道了一个弹性蛋白酶依赖的突变型SIV A/Sw/Sk-R345 V（R345 V），它来源于A/Sw/Saskatchewan/18789/02（H1N1）（SIV/Sk 02），在猪中高度减毒。R345 V的两次腹膜内给药诱导了强烈的细胞介导和体液免疫应答，并对猪中抗原性不同的SIV感染提供了高度的保护。在这里，我们评估了免疫原性和保护效力的R345 V对SIV感染的鼻内给药，更实用的途径接种猪在现场。我们的数据表明，鼻内接种的R345 V活疫苗能够在两次应用后诱导局部气管-支气管淋巴细胞的强抗原特异性IFN-γ应答以及血清和呼吸道粘膜中的抗体应答。鼻内接种R345 V不仅对亲本野生型病毒感染，而且对同源抗原变体A/Sw/印第安纳州/1726/88（H1N1）感染提供了完全保护。此外，鼻内施用R345 V赋予部分保护免受猪中的异源亚型H3 N2 SIV感染。因此。R345 V弹性蛋白酶依赖性突变SIV可以作为针对猪中抗原性不同的猪流感病毒的活疫苗。(C)2010爱思唯尔有限公司保留所有权利。
Influenza A virus is an important respiratory pathogen of swine that causes significant morbidity and economic impact on the swine industry. Vaccination is the first choice for prevention and control of influenza infections. Live attenuated influenza vaccines (LAIV) are approved for use in humans and horses and their application provides broad protective immunity, however no LAIV against swine influenza virus (SW) exists in the market. Previously we reported that an elastase-dependant mutant SIV A/Sw/Sk-R345V (R345V) derived from A/Sw/Saskatchewan/18789/02 (H1N1) (SIV/Sk02) is highly attenuated in pigs. Two intratracheal administrations of R345V induced strong cell-mediated and humoral immune responses and provided a high degree of protection to antigenically different SIV infection in pigs. Here we evaluated the immunogenicity and the protective efficacy of R345V against SIV infection by intranasal administration, the more practical route for vaccination of pigs in the field. Our data showed that intranasally administered R345V live vaccine is capable of inducing strong antigen-specific IFN-gamma response from local tracheo-bronchial lymphocytes and antibody responses in serum and respiratory mucosa after two applications. Intranasal vaccination of R345V provided pigs with complete protection not only from parental wild type virus infection, but also from homologous antigenic variant A/Sw/Indiana/1726/88 (H1N1) infection. Moreover, intranasal administration of R345V conferred partial protection from heterologous subtypic H3N2 SIV infection in pigs. Thus. R345V elastase-dependent mutant SIV can serve as a live vaccine against antigenically different swine influenza viruses in pigs. (C) 2010 Elsevier Ltd. All rights reserved.