Microarray-based determination of anti-inflammatory genes targeted by 6-(methylsulfinyl)hexyl isothiocyanate in macrophages
Microarray-based determination of anti-inflammatory genes targeted by 6-(methylsulfinyl)hexyl isothiocyanate in macrophages
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DOI：
10.3892/etm_00000006
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发表时间：
2010-01-01
影响因子：
2.7
通讯作者：
Hou, De-Xing
中科院分区：
文献类型：
--
作者：
Chen, Jihua;Uto, Takuhiro;Hou, De-Xing
6-（甲基亚磺酰基）己基异硫氰酸酯（6-MSITC）是日本流行的辛辣香料山葵[Wasabia japonica（Miq）Matsumura]的生物活性成分。为了评价6-MSITC的抗炎功能和潜在的靶向基因，在小鼠巨噬细胞中通过DNA微阵列进行基因表达谱分析。在22，050个寡核苷酸中，在脂多糖（LPS）激活的RAW 264细胞中，406个基因的表达水平增加了≥ 3倍，其中238个基因信号被6-MSITC减弱（>= 2倍）在LPS激活的细胞中，717个基因的表达水平降低了>= 3倍，其中336个基因信号被6-MSITC恢复（>= 2倍）利用组分析，将受6-MSITC影响的具有>= 2倍变化的206个基因分为与生物过程（81个）、分子功能（108个）和信号传导途径（17个）相关的35类。免疫途径分析进一步揭示了Wasabi 6-MSITC通过调节趋化因子、白细胞介素和干扰素的相关网络发挥其抗炎作用
6-(Methylsulfinyl)hexyl isothiocyanate (6-MSITC) is a bioactive ingredient of wasabi [Wasabia japonica (Miq) Matsumura], which is a popular pungent spice of Japan To evaluate the anti-inflammatory function and underlying genes targeted by 6-MSITC, gene expression profiling through DNA microarray was performed in mouse macrophages Among 22,050 oligonucleotides, the expression levels of 406 genes were increased by >= 3-fold in lipopolysaccharide (LPS)-activated RAW264 cells, 238 gene signals of which were attenuated by 6-MSITC (>= 2-fold) Expression levels of 717 genes were decreased by >= 3-fold in LPS-activated cells, of which 336 gene signals were restored by 6-MSITC (>= 2-fold) Utilizing group analysis, 206 genes affected by 6-MSITC with a >= 2-fold change were classified into 35 categories relating to biological processes (81), molecular functions (108) and signaling pathways (17) The genes were further categorized as 'defense, inflammatory response, cytokine activities and receptor activities' and some were confirmed by real-time polymerase chain reaction Ingenuity pathway analysis further revealed that wasabi 6-MSITC regulated the relevant networks of chemokines, interleukins and interferons to exert its anti-inflammatory function