Characterization of putative toxin/antitoxin systems in Vibrio parahaemolyticus
Characterization of putative toxin/antitoxin systems in Vibrio parahaemolyticus
复制标题
DOI：
10.1111/jam.12513
复制
发表时间：
2014-07-01
影响因子：
4
通讯作者：
Kimura, M.
中科院分区：
文献类型：
--
作者：
Hino, M.;Zhang, J.;Kimura, M.
目的：为了获得更多关于弧菌属中毒素/抗毒素（TA）系统的信息，也为了研究它们参与诱导可存活但不可培养（VBNC）状态，我们在副溶血弧菌基因组中搜索了大肠杆菌TA系统的同源物。方法和结果：我们发现副溶血性弧菌基因组数据库中的一个基因簇vp 1842/vp 1843与编码大肠杆菌的基因簇具有同源性。coliTA蛋白，DinJ/YafQ.毒素基因vp 1843在E.大肠杆菌细胞强烈抑制细胞生长，而与推定的抗毒素基因VP 1842的共表达中和了这种作用。突变分析表明vp 1843基因产物VP 1843中的Lys 37和Pro45是导致大肠杆菌生长迟缓的关键残基。coli细胞。VP 1843与E.大肠杆菌毒素YafQ对蛋白质合成没有抑制活性，而vp 1843在大肠杆菌中的表达，结论：VP 1842/VP 1843基因簇编码副溶血性弧菌TA系统，VP 1843抑制细胞生长，VP 1842通过与VP 1843形成稳定的复合物发挥抗毒素作用。推测的毒素VP 1843可能通过抑制细胞分裂参与诱导副溶血性弧菌的VBNC状态。
Aim: To obtain more information about the toxin/antitoxin (TA) systems in the Vibrio genus and also to examine their involvement in the induction of a viable but nonculturable (VBNC) state, we searched homologues of the Escherichia coli TA systems in the Vibrio parahaemolyticus genome.Methods and Results: We found that a gene cluster, vp1842/vp1843, in the V. parahaemolyticus genome database has homology to that encoding the E. coli TA proteins, DinJ/YafQ. Expression of the putative toxin gene vp1843 in E. coli cells strongly inhibited the cell growth, while coexpression with the putative antitoxin gene vp1842 neutralized this effect. Mutational analysis identified Lys37 and Pro45 in the gene product VP1843 of vp1843 as crucial residues for the growth retardation of E. coli cells. VP1843, unlike the E. coli toxin YafQ, has no protein synthesis inhibitory activity, and that instead the expression of vp1843 in E. coli caused morphological change of the cells.Conclusions: The gene cluster vp1842/vp1843 encodes the V. parahaemolyticus TA system; VP1843 inhibits cell growth, whereas VP1842 serves as an antitoxin by forming a stable complex with VP1843.Significance and Impact of the Study: The putative toxin, VP1843, may be involved in the induction of the VBNC state in V. parahaemolyticus by inhibiting cell division.