The Bvg virulence control system regulates biofilm formation in Bordetella bronchiseptica
The Bvg virulence control system regulates biofilm formation in Bordetella bronchiseptica
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DOI：
10.1128/jb.186.17.5692-5698.2004
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发表时间：
2004-09-01
影响因子：
3.2
通讯作者：
Yuk, MH
中科院分区：
文献类型：
--
作者：
Irie, Y;Mattoo, S;Yuk, MH
波氏杆菌利用BvgAS(波氏杆菌毒力基因)双组分信号转导系统在至少三个阶段中感知环境和调节基因表达：毒力BVG(+)阶段、无毒BVG(-)阶段和中间BVG(I)阶段。在BVG(+)期表达的基因编码已知的毒力因子，包括粘附素，如丝状血凝素(FHA)和菌毛，以及毒素，如双功能腺苷环化酶/溶血素(Acy)。以往的研究表明，在BVG(I)期，FHA和菌毛继续表达，但Acy的表达显著下调。在这份报告中，我们确定了支气管败血波尔德氏菌在体外可以形成生物膜，并且在BVG(I)阶段生物膜的生成最多。我们表明，FHA是形成最大生物膜所必需的，菌毛也可能对这种表型有贡献。然而，Acy的表达抑制了生物膜的形成，很可能是通过与FHA的相互作用实现的。因此，粘附素和Acy表达的协同调控导致了BVG(I)期支气管败血杆菌生物膜的最大形成。
Bordetella species utilize the BvgAS (Bordetella virulence gene) two-component signal transduction system to sense the environment and regulate gene expression among at least three phases: a virulent Bvg(+) phase, a nonvirulent Bvg(-) phase, and an intermediate Bvg(i) phase. Genes expressed in the Bvg(+) phase encode known virulence factors, including adhesins such as filamentous hemagglutinin (FHA) and fimbriae, as well as toxins such as the bifunctional adenylate cyclase/hemolysin (ACY). Previous studies showed that in the Bvg(i) phase, FHA and fimbriae continue to be expressed, but ACY expression is significantly downregulated. In this report, we determine that Bordetella bronchiseptica can form biofilms in vitro and that the generation of biofilm is maximal in the Bvg(i) phase. We show that FHA is required for maximal biofilm formation and that fimbriae may also contribute to this phenotype. However, expression of ACY inhibits biofilm formation, most likely via interactions with FHA. Therefore, the coordinated regulation of adhesins and ACY expression leads to maximal biofilm formation in the Bvg(i) phase in B. bronchiseptica.