DECREASED RESISTANCE TO ANTIBIOTICS AND PLASMID LOSS IN PLASMID-CARRYING STRAINS OF STAPHYLOCOCCUS-AUREUS TREATED WITH ASCORBIC-ACID
DECREASED RESISTANCE TO ANTIBIOTICS AND PLASMID LOSS IN PLASMID-CARRYING STRAINS OF STAPHYLOCOCCUS-AUREUS TREATED WITH ASCORBIC-ACID
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DOI：
10.1016/0165-7992(91)90128-q
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发表时间：
1991-11-01
期刊：
影响因子：
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通讯作者：
WAHLABORDE, ME
中科院分区：
文献类型：
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作者：
AMABILECUEVAS, CF;PINAZENTELLA, RM;WAHLABORDE, ME
研究了抗坏血酸对金黄色葡萄球菌质粒编码抗生素耐药性的影响。几株S.金黄色葡萄球菌在1 mM抗坏血酸存在下培养6小时。这种治疗诱导的6个菌株中的4个测试的抗性标记的损失增加，琼脂糖凝胶电泳显示抗坏血酸诱导的易感菌落中的质粒DNA的消失。抗坏血酸盐的存在下，不同的抗生素的耐药菌株的最小抑菌浓度降低50-75%。当添加抗坏血酸盐时，以前亚抑制浓度的青霉素或四环素对耐药菌株的抑制作用增强，甚至导致25-93%的初始种群死亡。这些结果表明，抗坏血酸可诱导S.金黄色葡萄球菌，抗生素耐药性的水平也受到这种化合物的存在的影响。
The effect of ascorbic acid on plasmid-coded antibiotic resistance in Staphylococcus aureus was investigated. Several strains of S. aureus were cultured in the presence of 1 mM ascorbate for 6 h. This treatment induced an increased loss of resistance markers in 4 of 6 strains tested, and agarose gel electrophoresis showed this disappearance of plasmid DNA in ascorbate-induced susceptible colonies. The presence of ascorbate induced a 50-75% decrease in minimal inhibitory concentrations of different antibiotics for resistant strains. When ascorbate is added, formerly subinhibitory concentrations of penicillin or tetracycline have an increased inhibitory effect on resistant strains and even induced the death of 25-93% of the intial population. These results suggest that ascorbate can induce the loss of several plasmids of S. aureus, and that the levels of antibiotic resistance are also affected by the presence of this compound.