Field evaluation of an enzyme-linked immunosorbent assay (ELISA) for Plasmodium falciparum sporozoite detection in anopheline mosquitoes from Kenya.
Field evaluation of an enzyme-linked immunosorbent assay (ELISA) for Plasmodium falciparum sporozoite detection in anopheline mosquitoes from Kenya.
复制标题
对肯尼亚按蚊中恶性疟原虫子孢子检测的酶联免疫吸附测定 (ELISA) 进行现场评估。
DOI：
--
复制
发表时间：
1987
影响因子：
3.3
通讯作者：
W. Hockmeyer
中科院分区：
文献类型：
--
作者：
J. Beier;P. Perkins;R. Wirtz;R. Whitmire;M. Mugambi;W. Hockmeyer
肯尼亚使用酶联免疫吸附测定 (ELISA) 来评估冈比亚按蚊的疟疾感染，该测定使用单克隆抗体来识别恶性疟原虫环子孢子蛋白上的重复表位。和安。富内斯图斯。 ELISA 证实 44 个子孢子阳性腺体解剖中 88% 是恶性疟原虫。单独测试的两个物种的蚊子的 ELISA 感染率为 18.6% (n = 736)，显着高于通过解剖测定的 10.4% (n = 537) 唾液腺子孢子率。这种差异是由于 ELISA 检测到受感染蚊子中肠上的中型和大型卵囊，其中不含唾液腺子孢子。从一系列从胸部切开的 379 只按蚊中，对“头部”和“身体”部分进行 ELISA 测试表明，在没有唾液腺感染的情况下，95 只阳性蚊子中有 29.5% 的身体部分含有环子孢子抗原。该现场评估表明，通过切割蚊子的胸部并测试前部，可以最准确地使用 ELISA 来估计子孢子率。
An enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody that recognizes a repetitive epitope on the circumsporozoite protein of Plasmodium falciparum was used in Kenya to assess malaria infections in Anopheles gambiae s.l. and An. funestus. The ELISA confirmed that 88% of 44 sporozoite-positive gland dissections were P. falciparum. The ELISA infection rate of 18.6% (n = 736) for individually tested mosquitoes for both species was significantly higher than the 10.4% (n = 537) salivary gland sporozoite rate determined by dissection. This difference was due to ELISA detection of medium and large sized oocysts on the midguts of infected mosquitoes which did not contain salivary gland sporozoites. From a series of 379 Anopheles that were cut at the thorax, ELISA tests on "head" and "body" portions showed that 29.5% of 95 positive mosquitoes contained circumsporozoite antigen in the body portion in the absence of salivary gland infections. This field evaluation demonstrates that the ELISA can most accurately be used to estimate sporozoite rates by cutting mosquitoes at the thorax and testing anterior portions.