A postulated mechanism for the coordinate effects of ionophore A23187 on calcium uptake and cell viability in rat thymocytes.
A postulated mechanism for the coordinate effects of ionophore A23187 on calcium uptake and cell viability in rat thymocytes.
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离子载体 A23187 对大鼠胸腺细胞钙摄取和细胞活力协调作用的假设机制。
DOI：
10.1016/0005-2736(82)90042-6
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Ingbar,SH
中科院分区：
文献类型：
--
作者：
Segal,J;Ingbar,SH
在钙离子的生理浓度下，推测的钙离子载体A23187引起大鼠胸腺细胞摄取45Ca的剂量相关增加和细胞存活率的下降，这一效应显示出很强的线性相关性。相反，在钙离子浓度很低(7·10−6M)的培养液中，离子载体A23187对~(45)Ca的摄取和细胞存活率均无特异性影响。离子载体A23187的钙依赖细胞毒性类似于其他非离子载体的药物，但已知这些药物会扰乱质膜。因此，我们认为，在大鼠胸腺细胞中，离子载体A23187可能不是真正的离子载体，而是可能扰乱细胞膜，允许钙离子沿着其电化学梯度自由通过细胞膜。
At the physiological concentration of Ca2+, the presumed calcium ionophore A23187 produced dose-related increases in45Ca uptake by rat thymocytes and decreases in cell viability, effects that displayed a strong linear correlation. In media containing a very low concentration of Ca2+(7 · 10−6M), in contrast, ionophore A23187 had no specific effect on either45Ca uptake or cell viability. The calcium-dependent cytotoxicity of ionophore A23187 resembles that of other agents that are not ionophores, but that are known to perturb the plasma membrane. Consequently, we suggest that, in the rat thymocyte, ionophore A23187 may not act as a true ionophore, but may perturb the cell membrane, allowing Ca2+to pass freely through the membrane along its electrochemical gradient.