Oleate stimulation of diacylglycerol formation from phosphatidylcholine through effects on phospholipase D and phosphatidate phosphohydrolase.
Oleate stimulation of diacylglycerol formation from phosphatidylcholine through effects on phospholipase D and phosphatidate phosphohydrolase.
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油酸通过影响磷脂酶 D 和磷脂酸磷酸水解酶刺激磷脂酰胆碱形成二酰甘油。
DOI：
10.1111/j.1432-1033.1992.tb17460.x
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Exton,JH
中科院分区：
文献类型：
--
作者：
Siddiqui,RA;Exton,JH
外源性磷脂酰胆碱（PtdCho）的水解为1，2-二酰甘油的大鼠肝质膜刺激油酸浓度低至0.1 mM。在75 mM乙醇的存在下，脂肪酸也增强磷脂酰乙醇（PtdEtOH）形成PtdCho。亚油酸和花生四烯酸也观察到了这些影响，但饱和脂肪酸或去污剂没有观察到这些影响，并且在微粒体或线粒体中最小。油酸可刺激外源性Ptd[~ 3 H]Cho释放[~ 3 H]胆碱，而抑制磷酸化[~ 3 H]胆碱的形成。油酸和其他不饱和但不饱和的脂肪酸也刺激外源性[14 C]磷脂酸转化为[14 C]二酰基甘油。这些数据与这些脂肪酸对肝质膜中磷脂酶D和磷脂酸磷酸水解酶的刺激作用一致。鸟苷5′-O-[3-硫代]三磷酸（20 μM）对PtdCho生成PtdEtOH和甘油二酯的刺激作用可通过低浓度油酸酯增强。磷脂酶A2也刺激PtdCho生成PtdEtOH和甘油二酯。不饱和脂肪酸可能通过激活磷脂酶D和磷脂酸磷酸水解酶来调节二酰甘油的产生。
Hydrolysis of exogenous phosphatidylcholine (PtdCho) to 1,2‐diacylglycerol by rat liver plasma membranes was stimulated by oleate concentrations as low as 0.1 mM. In the presence of 75 mM ethanol, the fatty acid also enhanced phosphatidylethanol (PtdEtOH) formation from PtdCho. These effects were also observed with linoleate and arachidonate, but not with saturated fatty acids or detergents, and were minimal in microsomes or mitochondria. Release of [3H]choline form exogenous Ptd[3H]Cho was stimulated by oleate, whereas phosphory1[3H]choline formation was inhibited. Oleate and other unsaturated, but not saturated, fatty acids also stimulated the conversion of exogenous [14C]phosphatidic acid to [14C]diacylglycerol. These data are consistent with stimulatory effects of these fatty acids on both phospholipase D and phosphatidate phosphohydrolase in liver plasma membranes. The stimulatory effect of guanosine 5′‐O‐[3‐thio]triphosphate) (20 μM) on PtdEtOH and diacylglycerol formation from PtdCho was enhanced by low concentration of oleate. Phospholipase A2also stimulated PtdEtOH and diacylglycerol formation from exogenous PtdCho. It is proposed that unsaturated fatty acids may play a physiological role in the regulation of diacylglycerol production through activation of phospholipase D and phosphatidate phosphohydrolase.