IMMUNOFLUORESCENT LOCALIZATION OF SCHISTOSOMA-MANSONI CIRCULATING CATHODIC ANTIGEN IN TISSUES OF INFECTED MICE USING MONOCLONAL-ANTIBODY
IMMUNOFLUORESCENT LOCALIZATION OF SCHISTOSOMA-MANSONI CIRCULATING CATHODIC ANTIGEN IN TISSUES OF INFECTED MICE USING MONOCLONAL-ANTIBODY
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DOI：
10.1007/bf00928334
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发表时间：
1985-01-01
期刊：
影响因子：
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通讯作者：
QIAN, ZL
中科院分区：
文献类型：
--
作者：
DEELDER, AM;ELDOSOKY, I;QIAN, ZL
The kinetics of the uptake and deposition of the major circulating cathodic antigen (CCA) of S. mansoni in liver, spleen, and kidney of S. mansoni infected Swiss mice was investigated in relation to the duration of infection and infection dose (50, 100, 200 cercariae). The presence of antigen was studied with a direct immunofluorescence reaction on frozen sections of the mouse organs, using a fluorescein isothiocyanate (FITC)-labeled mouse IgM monoclonal antibody recognizing a repeating epitope of CCA. CCA was demonstrable from 2 wk post infection (p.i.) onwards in Kupffer cells in the liver from 3-4 wk p.i. onwards in macrophages in the marginal zones in the spleen and from 8 wk p.i. onwards in kidney glomeruli. The immunofluorescence reactions on CCA in kidney glomeruli remained relatively weak.
