Effects of o,p′-DDE, heptachlor, and 17β-estradiol on vitellogenin gene expression and the growth hormone/insulin-like growth factor-I axis in the tilapia, Oreochromis mossambicus
Effects of o,p′-DDE, heptachlor, and 17β-estradiol on vitellogenin gene expression and the growth hormone/insulin-like growth factor-I axis in the tilapia, Oreochromis mossambicus
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DOI：
10.1016/j.cbpc.2008.11.007
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发表时间：
2009-05-01
影响因子：
3.9
通讯作者：
Grau, E. Gordon
中科院分区：
文献类型：
--
作者：
Davis, Lori K.;Visitacion, Nancy;Grau, E. Gordon
研究了两种内分泌干扰物o、p′-DDE和七氯酚以及17 -雌二醇（EA）对雄性罗非鱼卵黄原素（Vg）和生长激素(GH)/胰岛素样生长因子- i （IGF-I）轴的影响。在第一个实验中，每周给鱼注射5次E-2、o、p'-DDE或七肽（5 μ g/g）。E-2处理增加了血浆Vg和肝脏3个Vg基因（Vgs A、b和C）和雌激素受体α (ER α)的表达，同时降低了血浆IGF-I水平，抑制了IGF-I、GH受体（GHR2）和推测的生长肌动素受体（GHR1）的表达。除了显著降低GHR2的表达和增加血浆IGF-I外，这两种农药对其他参数的影响都不大。在第二个实验中，给鱼单次注射o，p'-DDE或七氯胺（100 μ g/g）或E-2 (5 μ g/g)，并在注射后5天处死。E2刺激了所有三个Vg基因的表达。o、p′-DDE和七氯胺均能增加VgB的表达，而只有o、p′-DDE能增加VgA的表达。o、p'-DDE和七氯胺对VgC表达和血浆Vg水平均无影响。0,p'-DDE和七氯以及E-2治疗增加了ER α和ER β转录物水平。同样，这两种农药都增加了GHR1和IGF-I的表达，而E-2对GHR1、GHR2和IGF-I的表达没有显著影响。这些结果表明，o,p'-DDE和七氯对Vg和GH/IGF-I轴的调制具有不同的时间和剂量效应，与E-2不同。(c) 2008爱思唯尔公司版权所有。
Effects of two endocrine disruptors, o,p'-DDE and heptachlor, and 17 beta-estradiol (EA on vitellogenin (Vg) and the growth hormone (GH)/insulin-like growth factor-I (IGF-I) axis were examined in male tilapia. In the first experiment, fish were given 5 weekly injections of either E-2, o,p'-DDE or heptachior (5 mu g/g). E-2 treatment increased plasma Vg and hepatic expression of three Vg genes (Vgs A, B. and C) and estrogen receptor alpha (ER alpha), while reducing plasma levels of IGF-I and Suppressing the expression of IGF-I, the GH receptor (GHR2) and the putative somatolactin receptor (GHR1). Neither pesticide greatly affected the other parameters examined, except for a significant reduction in expression of GHR2 and increased plasma IGF-I. In the second experiment, fish were given a single injection of o,p'-DDE or heptachlor (100 mu g/g), or E-2 (5 mu g/g) and sacrificed 5 days post-injection. Treatment with E2 Stimulated expression of all three Vg genes. Both o,p'-DDE and heptachlor increased expression of VgB, whereas only o,p'-DDE increased VgA expression. There was no effect of o,p'-DDE or heptachlor on VgC expression or plasma Vg levels. Treatment with o,p'-DDE and heptachlor as well as E-2 increased ER alpha and ER beta transcript levels. Similarly, both pesticides increased GHR1 and IGF-I expression, whereas no significant effect of E-2 was observed on GHR1, GHR2 or IGF-I expression. These results indicate that o,p'-DDE and heptachlor have varying temporal and dose effects on modulation of Vg and the GH/IGF-I axis that are distinct from E-2. (c) 2008 Elsevier Inc. All rights reserved.