Investigation of spatial and temporal variation of particulate matter in vitro genotoxicity and cytotoxicity in relation to the elemental composition
Investigation of spatial and temporal variation of particulate matter in vitro genotoxicity and cytotoxicity in relation to the elemental composition
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DOI：
10.1016/j.mrgentox.2019.01.009
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发表时间：
2019-06-01
影响因子：
1.9
通讯作者：
Gaga, Eftade O.
中科院分区：
文献类型：
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作者：
Cakmak, Gonca;Ari, Pelin Erturk;Gaga, Eftade O.
尽管室外空气污染及其主要成分颗粒物（PM）最近被列为人类致癌物，试图阐明PM毒性的潜在机制仍然是至关重要的，并继续在各种环境条件下的体外方法。本研究调查了遗传毒性（彗星试验）和细胞毒性（乳酸脱氢酶（LDH）泄漏和水溶性四唑（WST-1）测定）的30个每日PM2.5样本收集在库塔亚省，以解决他们的影响与季节的每日变化（即冬季与夏季）和地点（即农村与城市）使用A549人肺癌上皮细胞系，以及与其化学成分，特别是微量元素，有机碳（OC）和元素碳（EC）。的遗传毒性，测量的百分比尾强度（TI），在交通密集的城市站的每日PM2.5样品是高于农村网站的80%的平行天。在城市的冬季和农村的夏季，遗传毒性是显着的。冬季城市样品的细胞毒性最高。PM2.5质量、OC和EC浓度与DNA损伤不相关，而Mn、Fe、Cu和Ba在农村PM2.5样品中与DNA损伤相关，Mn、Co和Ni在城市样品中与DNA损伤相关。目前的研究证实，PM2.5的复杂组成来源于空间和时间的变化，可以导致健康影响的差异。
Even though the outdoor air pollution and its major component Particulate Matter (PM) are recently classified as human carcinogen, attempts to elucidate the underlying mechanisms of PM toxicity are still crucial and continuing with in vitro approaches in various environmental circumstances. Present study investigated the genotoxicity (Comet assay) and the cytotoxicity (lactate dehydrogenase (LDH) leakage and the water-soluble tetrazolium (WST-1) assays) of 30 daily PM2.5 samples collected in the Kutahya province, to address their daily variability in effects with season (i.e. winter versus summer) and location (i.e. rural versus urban) using A549 human lung cancer epithelial cell line, as well as in relation to their chemical composition, specifically trace elements, organic carbon (OC) and elemental carbon (EC). The genotoxicity, measured by the percentage tail intensity (TI), of the daily PM2.5 samples at the traffic dense urban station was higher than that of the rural site for 80% of the parallel days. The genotoxicity was significant in the winter at the urban and in the summer at the rural site. Cytotoxicity was the highest for the winter urban samples. The PM2.5 mass, OC, and EC concentrations were not correlated to DNA damage, while there were correlations with Mn, Fe, Cu and Ba at the rural PM2.5 samples, and Mn, Co and Ni at the urban samples, respectively. The present study is confirming that the complex composition of PM2.5 originating from spatial and temporal changes can cause differences in the health effects.