LEUKOTRIENE-B4 INDUCES INVITRO FIBROBLAST CHEMOTAXIS
LEUKOTRIENE-B4 INDUCES INVITRO FIBROBLAST CHEMOTAXIS
复制标题
DOI：
10.1111/1523-1747.ep12258678
复制
发表时间：
1984-01-01
影响因子：
6.5
通讯作者：
CZARNETZKI, BM
中科院分区：
文献类型：
--
作者：
MENSING, H;CZARNETZKI, BM
在体外成纤维细胞趋化性进行了研究，在存在合成的白三烯B4（LTB 4）和离子载体刺激的淋巴细胞，单核细胞和嗜碱性粒细胞（LMB-S）的上清液。LTB 4和LMB-S诱导人和大鼠胚胎成纤维细胞的剂量相关的定向迁移。通过棋盘格分析，这种反应是趋化性的，而不是趋化动力学的。在10-8 M浓度下发生向LTB 4的最佳迁移，而更高浓度则具有抑制作用。LMB-S含有2种类型的成纤维细胞趋化因子，一种对应于LTB 4，另一种为> 10千道尔顿的热不稳定、不可透析物质。LTB 4和LMB-S对转化的人结缔组织细胞（[肉瘤] HT 1080，[滑液]麦考伊）无活性，而这些细胞对来自融合成纤维细胞单层培养物的条件培养基反应良好。LTB 4是成纤维细胞的特异性化学引诱剂，它与来自炎症白细胞的其他趋化因子协同作用，调节成纤维细胞向组织部位的流入。
In vitro fibroblast chemotaxis was studied in the presence of synthetic leukotriene B4 (LTB4) and of supernatants from ionophore-stimulated lymphocytes, monocytes and basophils (LMB-S). LTB4 and LMB-S induced a dose-related, directed migration of human and rat embryonic fibroblasts. By checkerboard analysis, this response was chemotactic and not chemokinetic. Optimal migration toward LTB4 occurred at 10-8 M concentrations, while higher concentrations were inhibitory. The LMB-S contained 2 types of fibroblast chemotactic factors, one that corresponded to LTB4 and one additional, heat labile, non-dialyzable substance of > 10 kilodaltons. LTB4 and LMB-S were inactive against transformed human connective tissue cells ([sarcoma] HT 1080, [synovial fluid] McCoy) while these cells moved well in response to conditioned medium derived from confluent fibroblast monolayer cultures. LTB4 is a specific chemoattractant for fibroblasts and it acts in concert with other chemotactic factors derived from inflammatory leukocytes to regulate the influx of fibroblasts to tissue sites.