Genetics of the tubulin gene families of Physarum.
Genetics of the tubulin gene families of Physarum.
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绒泡菌微管蛋白基因家族的遗传学。
DOI：
10.1093/genetics/108.1.143
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发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
Burland,TG
中科院分区：
文献类型：
--
作者：
Schedl,T;Owens,J;Dove,WF;Burland,TG
通过孟德尔分析研究了绒泡菌中α-和β-微管蛋白基因家族的组织。限制性核酸内切酶生成的与 α- 和 β- 微管蛋白同源的 DNA 片段显示出长度多态性，可用作遗传图谱的标记。对杂合子后代减数分裂分类的分析允许定义α-和β-微管蛋白序列位点。有四个未连锁的 α-微管蛋白序列基因座（altA、altB、altCandaltD）和至少三个未连锁的 β-微管蛋白序列基因座（betA、betBandbetC）。 α-微管蛋白基因座与 β-微管蛋白基因座无关。 — 微管蛋白序列基因座与赋予抗微管蛋白苯并咪唑药物抗性的 toben 突变相关的分离用于研究 α- 或 β- 微管蛋白基因家族的任何成员是否是等位基因 toben 基因座。 β-微管蛋白序列基因座betBi与抗性基因座D等位，betA基因座可能是等位基因tobenA，并且α-微管蛋白序列基因座tC可能是等位基因tobenC。当只有一个表达的β-微管蛋白基因家族成员突变为耐药性时，苯并咪唑耐药表型的分子含义与微管蛋白功能相关。
The organization of the α- and β-tubulin gene families in Physarum was investigated by Mendelian analysis. Restriction endonuclease-generated DNA fragments homologous to α- and β-tubulin show length polymorphisms that can be used as markers for genetic mapping. Analysis of meiotic assortment among progeny of heterozygotes allowed α- and β-tubulin sequence loci to be defined. There are four unlinked α-tubulin sequence loci (altA, altB, altCandaltD) and at least three unlinked β-tubulin sequence loci (betA, betBandbetC). The α-tubulin loci are not linked to the β-tubulin loci. —Segregation of tubulin sequence loci with respect tobenmutations that confer resistance to antitubulin benzimidazole drugs was used to investigate whether any members of the α- or β-tubulin gene families are allelic tobenloci. The β-tubulin sequence locusbetBis allelic to the resistance locusbenD, thebetAlocus is probably allelic tobenAand the α-tubulin sequence locusaltCmay be allelic tobenC. The molecular implications of benzimidazole resistance phenotypes when only one of the expressed β-tubulin gene family members mutates to drug resistance are discussed in relation to tubulin function.