PRIMARY STRUCTURE AND TRANSCRIPTIONAL REGULATION OF GAP-43, A PROTEIN ASSOCIATED WITH NERVE GROWTH
PRIMARY STRUCTURE AND TRANSCRIPTIONAL REGULATION OF GAP-43, A PROTEIN ASSOCIATED WITH NERVE GROWTH
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DOI：
10.1016/0092-8674(87)90616-7
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发表时间：
1987-06-19
期刊：
影响因子：
64.5
通讯作者：
SKENE, JHP
中科院分区：
文献类型：
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作者：
BASI, GS;JACOBSON, RD;SKENE, JHP
神经再生和轴突的发育生长都与称为GAP-43的轴突膜蛋白的合成增加相关。在成年人大脑中，一种明显相同的蛋白质的磷酸化水平较低，这与长时程增强（一种突触可塑性）有关。我们现在已经从新生大鼠脑中分离出编码GAP-43的cDNA克隆。氨基酸序列是非常亲水的，没有潜在的跨膜结构域，也没有N-连接糖基化的位点，但在蛋白质的氨基末端有一个短的疏水片段，这与GAP-43从生长锥和突触质膜的细胞质表面延伸的模型一致。在所检测的几种组织和细胞中，GAP-43 mRNA仅在神经元中表达。GAP-43合成的发育和再生相关变化似乎主要在单个基因的转录水平上介导。
Nerve regeneration and developmental outgrowth of axons are both correlated with increased synthesis of an axonal membrane protein designated GAP-43. Phosphorylation of an apparently identical protein, present at lower abundance in adult brains, has been correlated with long-term potentiation, a form of synaptic plasticity. We have now isolated a cDNA clone encoding GAP-43 from neonatal rat brain. The amino acid sequence is extremely hydrophilic, with no potential membrane-spanning domains and no sites for N-linked glycosylation, but with a short hydrophobic segment at the protein''s amino terminus, consistent with a model in which GAP-43 extends from the cytoplasmic surface of growth cone and synaptic plasma membranes. Among several tissues and cells examined, GAP-43 mRNA is expressed only in neurons. Developmental and regeneration-associated changes in GAP-43 synthesis appear to be mediated largely at the level of transcription of a single gene.