Peptide mass fingerprinting using MALDI-TOF mass spectrometry.
Peptide mass fingerprinting using MALDI-TOF mass spectrometry.
复制标题
使用 MALDI-TOF 质谱法进行肽质量指纹分析。
作者：
D. Pappin
常规操作的大幅面2D凝胶电泳仪能够在1或2天(1，2)内解析数千个细胞蛋白质。在过去的十年里，埃德曼微序列分析和特定抗体染色鉴定蛋白质的组合被用于系统鉴定蛋白质和建立细胞数据库(3-5)。然而，与这些方法相关的问题很多。大多数蛋白质只存在于低至上飞摩尔范围内，这大大低于自动测序仪能够可靠操作的水平(6，7)。埃德曼过程的相对较慢的速度也意味着蛋白质的数量太多，无法在任何有用的时间段内进行大规模的表征。使用单抗，虽然快速和敏感，但需要现成的大量特定抗体探针池。
Large-format 2D gel electrophoresis systems in routine operation are capable of resolving several thousand cellular proteins in 1 or 2 d (1,2). For the last decade, a combination of Edman microsequence analysis and identification of proteins by staining with specific antibodies has been used to systematically identify proteins and establish cellular databases (3–5). There are, however, significant problems associated with these approaches. Most proteins are only present in the low- to upper-femtomole range, which is significantly below the level at which automated sequencers can reliably operate (6,7). The relatively slow speed of the Edman process also means that the number of proteins is too great to permit large-scale characterization within any useful period of time. The use of monoclonal antibodies, while both rapid and sensitive, requires the ready availability of a large pool of specific antibody probes.
DOI：
10.1073/pnas.84.20.6970
发表时间：
1987-10-01
影响因子：
11.1
作者：
AEBERSOLD, RH;LEAVITT, J;KENT, SBH
通讯作者：
KENT, SBH
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Garrels,JI;FranzaJr,BR
通讯作者：
FranzaJr,BR