CLONING AND EXPRESSION OF A CDNA CODING FOR THE HUMAN PLATELET-DERIVED GROWTH-FACTOR RECEPTOR - EVIDENCE FOR MORE THAN ONE RECEPTOR CLASS
CLONING AND EXPRESSION OF A CDNA CODING FOR THE HUMAN PLATELET-DERIVED GROWTH-FACTOR RECEPTOR - EVIDENCE FOR MORE THAN ONE RECEPTOR CLASS
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DOI：
10.1073/pnas.85.10.3435
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发表时间：
1988-05-01
影响因子：
11.1
通讯作者：
MURRAY, MJ
中科院分区：
文献类型：
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作者：
GRONWALD, RGK;GRANT, FJ;MURRAY, MJ
提供了编码人血小板衍生生长因子(PDGF)受体的CDNA的完整核苷酸序列。该基因含有一个开放阅读框，编码1106个氨基酸的蛋白质。与小鼠PDGF受体的氨基酸序列同源性为86%。在胞质分裂酪氨酸激酶结构域中，该序列的同源性达到98%。人真皮成纤维细胞Poly(A)+RNA的RNA印迹杂交分析检测到一个主要的(.apprxeq.5.7kb)和一个次要的(.apprxeq.4.8kb)转录本。用含有受体基因的表达载体转染的新生仓鼠肾细胞，表达一种约190 kDa的细胞表面蛋白，该蛋白可被抗人PDGF受体识别，并通过配体诱导的受体磷酸化证明该蛋白在转基因的新生仓鼠肾细胞中具有功能。用纯的BB和AB亚型的PDGF(即由两个B链或一个A和B链组成的PDGF二聚体)来评价重组PDGF受体的结合特性。与人的皮肤成纤维细胞不同，小仓鼠肾脏细胞只与PDGF的BB亚型高亲和力结合。这一观察结果与不止一个PDGF受体类别的存在是一致的。
The complete nucleotide sequence of a CDNA encoding the human platelet-derived growth factor (PDGF) receptor is presented. The cDNA contains an open reading frame that codes for a protein of 1106 amino acids. Comparison to the mouse PDGF receptor reveals an overall amino acid sequence identity of 86%. This sequence identity rises to 98% in the cytoplasmic split tyrosine kinase domain. RNA blot hybridization analysis of poly (A)+ RNA from human dermal fibroblasts detects a major (.apprxeq.5.7 kb) and a minor (.apprxeq. 4.8 kb) transcript using the cDNA as a probe. Baby hamster kidney cells, transfected with an expression vector containing the receptor cDNA, express an .apprxeq.190-kDa cell surface protein that is recognized by an anti-human PDGF receptor is functional in the transfected baby hamster kidney cells as demonstrated by ligand-induced phosphorylation of the receptor. Binding properties of the recombinant PDGF receptor were also assessed with pure preparations of BB and AB isoforms of PDGF (i.e., PDGF dimers composed of two B chains or an A and a B chain). Unlike human dermal fibroblasts baby hamster kidney cells bind only the BB isoform of PDGF with high affinity. This observation is consistent with the existence of than one PDGF receptor class.