Characterization of human megakaryocyte colony-stimulating factor in the urinary extracts from patients with aplastic anemia and idiopathic thrombocytopenic purpura
Characterization of human megakaryocyte colony-stimulating factor in the urinary extracts from patients with aplastic anemia and idiopathic thrombocytopenic purpura
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再生障碍性贫血和特发性血小板减少性紫癜患者尿提取物中人巨核细胞集落刺激因子的表征
DOI：
10.1182/blood.v61.3.556.bloodjournal613556
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发表时间：
1983
期刊：
影响因子：
20.3
通讯作者：
T. Miyake
中科院分区：
文献类型：
--
作者：
M. Kawakita;M. Ogawa;E. Goldwasser;T. Miyake
使用的程序，是有效的人尿促红细胞生成素（Epo）的纯化，我们试图从再生障碍性贫血（AA）和特发性血小板减少性紫癜（ITP）患者的尿提取物中的巨核细胞集落刺激因子（CSF）的初步纯化。比较纯化的人Epo和尿粗提物的集落刺激，发现：（1）纯Epo增加培养物中巨核细胞集落的形成;（2）MEG-CSF活性也存在于两种患者尿粗提物中Epo以外的物质中。类似于Epo的纯化，乙醇沉淀和磺丙基葡聚糖凝胶色谱法分别提供了MEG-CSF比活性的两倍和三倍增加。然而，与Epo相反，用苯酚处理观察到MEG-CSF活性的显著失活。尿提取物的羟基磷灰石色谱上所见的MEG-CSF的洗脱曲线与Epo的洗脱曲线不同。这些数据为纯化MEG-CSF的初始步骤提供了基础，并支持MEG-CSF与Epo不同的观点。
Using procedures that were effective in the purification of human urinary erythropoietin (Epo), we attempted initial purification of megakaryocyte colony-stimulating factors (CSF) in urinary extracts from patients with aplastic anemia (AA) and idiopathic thrombocytopenic purpura (ITP). Comparison of colony stimulation by purified human Epo and crude urinary extracts revealed: (1) that the pure Epo augments megakaryocyte colony formation in culture and (2) MEG-CSF activity is also present in materials other than Epo in the crude urinary extracts from the two types of patients. Similar to purification of Epo, ethanol precipitation and sulfopropyl-Sephadex chromatography provided twofold and threefold increases in the specific activity of MEG-CSF, respectively. In contrast to Epo, however, significant inactivation of MEG-CSF activity was seen with phenol treatment. The elution profile of MEG-CSF seen on hydroxylapatite chromatography of urinary extracts was different from that of Epo. These data provided a basis for initial steps for purification of MEG-CSF and support the notion that MEG-CSF is distinct from Epo.