Nanoscale pipetting for controlled chemistry in small arrayed water droplets using a double-barrel pipet
Nanoscale pipetting for controlled chemistry in small arrayed water droplets using a double-barrel pipet
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DOI：
10.1021/nl052215i
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发表时间：
2006-02-01
期刊：
影响因子：
10.8
通讯作者：
Klenerman, D
中科院分区：
文献类型：
--
作者：
Rodolfa, KT;Bruckbauer, A;Klenerman, D
我们提出了一种新的方法，使当今化学和生物实验室中最常用的工具之一-微管小型化。我们已经使用玻璃制造的双桶纳米管可控地生产出体积小到有机层下几个阿特利特的阵列水滴。我们已经处理了单个液滴，并从吸管的一个桶中添加了额外体积或试剂的受控量。我们证明，这种方法可以用于小型化的无细胞蛋白表达。
We present a new methodology which provides for the miniaturization of one of the most common tools in use in chemistry and biology laboratories today-the micropipet. We have used glass-fabricated double-barrel nanopipets to controllably produce arrayed water droplets with volumes as small as a few attoliters under an organic layer. We have addressed individual droplets and added controlled amounts of either additional volume or reagents from one of the barrels of the pipet. We demonstrate that this method can be used for miniaturized cell-free protein expression.