Altered expression of CK7 and CK20 in preneoplastic and neoplastic lesions in ulcerative colitis
Altered expression of CK7 and CK20 in preneoplastic and neoplastic lesions in ulcerative colitis
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溃疡性结肠炎癌前病变和癌变病变中 CK7 和 CK20 表达的改变
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
R. Palmqvist
中科院分区：
文献类型：
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作者：
R. Stenling;J. Lindberg;J. Rutegård;R. Palmqvist
这项研究基于1977年开始的一项仍在进行的结肠镜检查监测计划中，来自瑞典北部一个确定的集水区的所有溃疡性结肠炎患者。从这些材料中，我们选择了八组患者的组织，包括正常对照活检(5)、非活动性结肠炎（10）、活动性结肠炎（10）、低级别发育不良（10）、高级别发育不良(6)、非整倍体（无发育不良和随后的发育不良）（10）和溃疡性结肠炎相关癌症(5)。通过免疫组化检测CK7和CK20的表达。正常对照和非活动性结肠炎的结肠黏膜CK7完全阴性。在10例活动性结肠炎患者中有9例，CK7以斑片状方式稀疏表达，并与活动性上皮炎症区相关。10例低级别发育不良患者中有7例CK7阳性，6例高级别发育不良患者中有3例。非整倍体未发育不良的样品完全阴性，而6例中有2例出现随后的发育不良。在这五种癌症中，有两种是CK7阳性。CK20几乎在所有样本中表达，但在肿瘤相关病变的隐窝下部相对较多。我们的研究结果表明CK7和CK20的表达可能与溃疡性结肠炎患者结肠黏膜肿瘤的发展有关。这些发现是否具有临床意义尚需进一步研究。
This study is based on all patients with ulcerative colitis from a defined catchment area in Northern Sweden in a still ongoing colonoscopy surveillance programme, which started in 1977. From this material we selected tissue from eight groups of patients consisting of normal control biopsies (5), inactive colitis (10), active colitis (10), findings of low‐grade dysplasia (10), high‐grade dysplasia (6), aneuploidy (without dysplasia and with subsequent dysplasia) (10), and ulcerative colitis‐associated cancers (5). The samples were evaluated according to immunohistochemical expression of CK7 and CK20. Colonic mucosa from normal controls and inactive colitis was found to be completely negative for CK7. In 9 out of 10 patients with active colitis, CK7 was sparsely expressed in a patchy manner and connected with active epithelial inflammatory areas. 7 out of 10 patients with low‐grade dysplasia and 3 out of 6 with high‐grade dysplasia were positive for CK7. Samples with aneuploidy without dysplasia were completely negative, while 2 out of 6 showing subsequent dysplasia were positive. Of the five cancers, two were positive for CK7. CK20 was expressed in nearly all samples but relatively more in the lower part of the crypts in neoplasia‐associated lesions. Our results indicate a possible relationship between expression of CK7 and CK20 and neoplastic development of colorectal mucosa in patients with ulcerative colitis. Further studies are needed to elucidate whether these findings have clinical significance.