N-glycoprotein surfaceome of human induced pluripotent stem cell derived hepatic endoderm.
N-glycoprotein surfaceome of human induced pluripotent stem cell derived hepatic endoderm.
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DOI：
10.1002/pmic.201600397
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发表时间：
2017-03
期刊：
影响因子：
3.4
通讯作者：
Gundry RL
中科院分区：
文献类型：
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作者：
Mallanna SK;Waas M;Duncan SA;Gundry RL
采用细胞表面捕获技术，对人诱导多能干细胞来源的肝内胚层细胞表面n -糖蛋白组进行了测定。共鉴定出395个细胞表面n -糖蛋白，其中n -糖肽为1273个。本研究确定了n -糖蛋白，这些n -糖蛋白不被预测定位于细胞表面，并提供了实验数据，有助于解决不明确或错误注释的跨膜拓扑注释。在概念验证分析中，将这些数据与其他细胞表面蛋白质组数据集相结合，有助于识别潜在的细胞类型和谱系限制标记和药物靶点，从而促进干细胞技术在机械发育研究、疾病建模、药物发现和再生医学中的应用。
Using Cell Surface Capture Technology, the cell surface N-glycoproteome of human induced pluripotent stem cell derived hepatic endoderm cells was assessed. Altogether, 395 cell surface N-glycoproteins were identified, represented by 1273 N-glycopeptides. This study identified N-glycoproteins that are not predicted to be localized to the cell surface and provides experimental data that assist in resolving ambiguous or incorrectly annotated transmembrane topology annotations. In a proof-of-concept analysis, combining these data with other cell surface proteome datasets is useful for identifying potentially cell type and lineage restricted markers and drug targets to advance the use of stem cell technologies for mechanistic developmental studies, disease modeling, drug discovery, and regenerative medicine.