One-Dimensional Protein-Based Nanoparticles Induce Lipid Bilayer Disruption: Carbon Nanotube Conjugates and Amyloid Fibrils
One-Dimensional Protein-Based Nanoparticles Induce Lipid Bilayer Disruption: Carbon Nanotube Conjugates and Amyloid Fibrils
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DOI：
10.1021/la103615b
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发表时间：
2010-11-16
期刊：
影响因子：
3.9
通讯作者：
Shiraki, Kentaro
中科院分区：
文献类型：
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作者：
Hirano, Atsushi;Uda, Ken;Shiraki, Kentaro
随着纳米技术的最新进展，人们越来越关注纳米粒子与细胞膜相互作用所产生的生物影响。纳米颗粒往往会吸附体内的蛋白质。因此，必须研究偶联物对细胞膜的物理性质，以阐明和评估它们的性质。我们研究了一维蛋白质纳米粒在生理盐水中是否会导致脂质体渗漏。碳纳米管与吸附的溶菌酶通过静电相互作用与脂质体相互作用，导致脂质体泄漏。令人惊讶的是，溶菌酶的淀粉样纤维在脂质体渗漏方面与结合物相似。本文描述的结果为纳米颗粒与细胞膜之间的相互作用提供了新的见解，包括它们的形状、机械性能和非共价相互作用。
Along with recent progress of nanotechnology, concern has risen about biological impacts of nanoparticles deriving from their interaction with cell membranes. Nanoparticles tend to adsorb proteins in vivo. Therefore, the physical properties of the conjugates to cell membranes must be investigated to elucidate and assess their properties. We examined whether one-dimensional protein-based nanoparticles induce liposome leakage in physiological saline. Carbon nanotube conjugates with adsorbed lysozyme interacted with the liposome through electrostatic interaction, leading to liposome leakage. Surprisingly, amyloid fibrils of lysozyme resembled the conjugate in terms of their effects on liposome leakage. Results described herein provide new insight into the interaction between nanoparticles and cell membranes in terms of their shape, mechanical properties, and noncovalent interactions.