Long-term in vitro culture of murine mast cells. III. Discrimination of mast cells growth factor and granulocyte-CSF.
Long-term in vitro culture of murine mast cells. III. Discrimination of mast cells growth factor and granulocyte-CSF.
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小鼠肥大细胞的长期体外培养。
DOI：
10.4049/jimmunol.129.3.1256
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发表时间：
1982
影响因子：
4.4
通讯作者：
M. Moore
中科院分区：
文献类型：
--
作者：
Y. Yung;M. Moore
鼠肥大细胞的长期体外生长依赖于存在肥大细胞生长因子（MCGF）的条件培养基中的有丝分裂原激活的脾白细胞或各种鼠白血病细胞系。MCGF与粒细胞集落刺激因子（G-CSF）有许多共同的特性。这两种因子都存在于由骨髓单核细胞白血病WEHI-3和T细胞淋巴瘤LBRM-33细胞系调节的培养基中。它们对胰蛋白酶处理相对敏感，并且耐煮沸温度。对WEHI-3衍生的G-CSF没有应答的NZB小鼠也对MCGF没有应答。然而，MCGF与G-CSF的不同之处在于对神经氨酸酶和乳铁蛋白的敏感性，乳铁蛋白是巨噬细胞CSF产生的抑制剂，其抑制WEHI-3细胞产生G-CSF而不影响MCGF的产生。此外，腹膜细胞产生G-CSF，但不MCGF时，用脂多糖刺激。在体外生产MCGF的正常脾细胞需要存在的T淋巴细胞，是相对巨噬细胞的独立性。本文对T细胞在肥大细胞成熟和生长中的作用以及MCGF的生理功能进行了讨论。
Long-term in vitro growth of murine mast cells was dependent on the presence of a mast cell growth factor (MCGF) present in media conditioned by mitogen-activated splenic leukocytes or by various murine leukemic cell lines. MCGF shared a number of properties with granulocyte colony-stimulating factor (G-CSF). Both factors were present in media conditioned by the myelomonocytic leukemic WEHI-3 and the T cell lymphoma, LBRM-33 cell lines. They were relatively sensitive to trypsin treatment, and were resistant to boiling temperature. NZB mice that failed to respond to WEHI-3-derived G-CSF also failed to respond to MCGF. MCGF differed from G-CSF, however, in sensitivity to neuraminidase and lactoferrin, an inhibitor of macrophage CSF production, suppressed G-CSF production by WEHI-3 cells without affecting MCGF production. Furthermore, peritoneal cells produced G-CSF but not MCGF when stimulated with lipopolysaccharide. In vitro production of MCGF by normal spleen cells required the presence of T lymphocytes and is relatively macrophage-independent. The role of T cells in the maturation and growth of mast cells and the physiologic function of MCGF are discussed.