A simple method for the parallel deep sequencing of full influenza A genomes
A simple method for the parallel deep sequencing of full influenza A genomes
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DOI：
10.1016/j.jviromet.2011.09.001
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发表时间：
2011-12-01
影响因子：
3.1
通讯作者：
Gilbert, M. Thomas P.
中科院分区：
文献类型：
--
作者：
Kampmann, Marie-Louise;Fordyce, Sarah L.;Gilbert, M. Thomas P.
鉴于甲型流感对人类和动物健康的主要威胁，及其通过突变和重配快速进化的能力，需要能够及时表征其特征的工具来帮助监测其进化和传播。为此，深度测序可能是一个非常有价值的工具。这项研究报告了一种综合方法，可以使用 Illumina Genome Analyzer IIx (GAIIx) 对甲型流感亚型的完整基因组进行深度测序。通过使用这种方法，在 GAIIx 流动池的单泳道上对 9 种病毒的完整基因组进行了并行测序，这些病毒代表 2009 年大流行的 H1N1 病毒、人类 H5N1 病毒和猪 H1N1 病毒，平均深度为 122 倍。该技术可应用于培养和未培养的病毒。 (C) 2011 Elsevier B.V. 保留所有权利。
Given the major threat of influenza A to human and animal health, and its ability to evolve rapidly through mutation and reassortment, tools that enable its timely characterization are necessary to help monitor its evolution and spread. For this purpose, deep sequencing can be a very valuable tool. This study reports a comprehensive method that enables deep sequencing of the complete genomes of influenza A subtypes using the Illumina Genome Analyzer IIx (GAIIx). By using this method, the complete genomes of nine viruses were sequenced in parallel, representing the 2009 pandemic H1N1 virus, H5N1 virus from human and H1N1 virus from swine, on a single lane of a GAIIx flow cell to an average depth of 122-fold. This technique can be applied to cultivated and uncultivated virus. (C) 2011 Elsevier B.V. All rights reserved.